Applications of LC/MS to Amino Acids, Peptides, and Proteins
The potential for application of liquid chromatography/mass spectrometry (LC/MS) to sequencing of peptides and proteins has long been recognized. This was among the earliest applications of the direct liquid introduction technique (DLI) pioneered by McLafferty and co-workers [75MC36, 76AR61]. Despite some early success, LC/MS was not applicable to larger peptides until the observation was made that thermospray could provide a “soft ionization” technique [80BL21] which yielded protonated molecules from peptides and other nonvolatile molecules without employing any external source of ionization. This new direct thermospray ionization process produced spectra very similar to those obtained by field desorption (FD), and subsequent work [83VE27] showed that a direct ion evaporation mechanism similar to that discussed earlier by Iribarne and Thomson [76IR47] was involved.
KeywordsHydrolysis HPLC Tyrosine Pyrolysis Trypsin
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