Summary
Polyacrylamide gel electrophoresis was used to examine several kinds of exoenzymes from a number of isolates of Aspergillus flavus, A. parasiticus, A. oryzae, A. sojae, A. tamarii and A. nomius. Enzymes studied were pectinases, ribonucleases, amylases and proteases. Methods used were similar to those previously published, with some additional media.
Very well defined patterns were obtained for each enzyme and substrate system. A. flavus, A. parasiticus, A. tamarii and A. nomius produced distinct patterns. However, A. oryzae produced patterns very similar to those of A. flavus, and A. sojae to those of A. parasiticus. Consequently, isoenzyme patterns could not be used to distinguish the domesticated species from their wild types.
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References
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© 1990 Springer Science+Business Media New York
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Cruickshank, R.H., Pitt, J.I. (1990). Isoenzyme Patterns in Aspergillus Flavus and Closely Related Species. In: Samson, R.A., Pitt, J.I. (eds) Modern Concepts in Penicillium and Aspergillus Classification. NATO ASI Series, vol 185. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3579-3_22
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DOI: https://doi.org/10.1007/978-1-4899-3579-3_22
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