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Identification of Pathogenic Bacteria by Headspace Gas Chromatography

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Analytical Microbiology Methods

Abstract

Gas Chromatographic (GC) analysis of metabolically produced alcohols, short and long chain fatty acids, and cell components provide precise criteria for the identification of bacteria.1,2 Generally, prior to GC analysis, volatile compounds are extracted from the growth broth and/or the washed bacteria. However, this extraction process often leads to the loss of some of the alcohols and short chain fatty acids because of their high volatility.3

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References

  1. R. S. Breed, E. G. D. Murray, and N. R. Smith, “Bergey’s Manual of Determinative Bacteriology”, 7th ed., Williams and Wilkins, Baltimore (1957).

    Google Scholar 

  2. L. V. Holdemann, E. Cato, and W. E. C. Moore, “Anaerobe Laboratory Manual”, 4th ed., Virginia Polytechnic Institute and State University, Blacksburg (1977).

    Google Scholar 

  3. H. S. H. Seifert, D. Hoffmann, and H. Boehnel, Differenzierung pathogener Clostridia mit Hilfe der Dampfraum-Gaschromatographie und statistischen Auswertung ihrer metaboliach gebildeten Fettsauremuster, Fortschr. Vet. Med. 37: 214 (1983).

    CAS  Google Scholar 

  4. S. Giercke-Sygusch, Untersuchungen zur Erstellung eines Atlas anaerober Bakterien, Diss. sc. agr. Gottingen (1987).

    Google Scholar 

  5. S. Giercke, H. Boehnel, H. S. H. Seifert, Atlas der Clostridia und Bacteroiides-Arten (in Vorbereitung), Universitaet Goettingen (1986).

    Google Scholar 

  6. D. Hoffmann, Differenzierung von Clostridien mit Hilfe der Dampfraum-Gaschromatographie und der statistischen Auswertung ihrer Fettsaeuremuster, Diss. sc. agr. Goettingen (1984).

    Google Scholar 

  7. B. Kolb, P. Pospisil, M. Jaklin, and D. Boege, Analytic methods for head-space analysis, Chromatogr. Newsletter 7: 1 (1979).

    CAS  Google Scholar 

  8. L. Larsson, P. A. Mardh, and G. Odham, Detection of alcohols and volatile fatty acids by head-space gas chromatography in identification of anaerobic bacteria, J. Clin. Microbiol. 7: 23 (1978).

    PubMed  CAS  Google Scholar 

  9. L. Larsson, P. A. Mardh, G. Odham, and M. L. Carlsson, Diagnosis of bacteriaemia by automated head-space capillary gas chromatography, J. Clin. Path. 35: 715 (1982).

    Article  PubMed  CAS  Google Scholar 

  10. H. S. H. Seifert, H. Boehnel, and A. Ranaivoson, Verhuetung von Anaerobeninfektionen bei Wiederkaeuern in Madagaskar durch intradermale Applikation von ultrafiltrierten Toxoiden standortspezifischer Clostridia, Dtsch. tierarztl. Wschr. 90: 274 (1983).

    CAS  Google Scholar 

  11. L. J. Turton, D. B. Drucker, L. A. Ganguli, and V. F. Hillier, Statistical significance of effect of growth medium on anaerobe fermentation products, J. Appl. Bact. 53: 30 (1982).

    Google Scholar 

  12. K. Rieke, Differenzierung von Clostridia mit Hilfe der Gaschromatographischen Analyse metabolisch gebildeter Fettsaeuren, Diss. sc. agr. Goettingen (1981).

    Google Scholar 

  13. P. Gonzalez-Salinas, Aetiologie und Inzidenz von Bodenseuchen im Nord-Osten Mexikos, Diss. sc. agr. Goettingen (in press).

    Google Scholar 

  14. J. Wuest, Schnelldiagnose anaerober Infektionen mit der direkten gaschromatographischen Untersuchung von klinischem Material, Schweiz. med. Wschr. 110: 362 (1980).

    Google Scholar 

  15. S. Heitefuss, to be published (1990).

    Google Scholar 

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Seifert, H.S.H., Giercke-Sygusch, S., Boehnel, H. (1990). Identification of Pathogenic Bacteria by Headspace Gas Chromatography. In: Fox, A., Morgan, S.L., Larsson, L., Odham, G. (eds) Analytical Microbiology Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3564-9_8

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  • DOI: https://doi.org/10.1007/978-1-4899-3564-9_8

  • Publisher Name: Springer, Boston, MA

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