Comparison of Different Methodological Approaches to the Study of the Erythrocyte NA+ - K+ Pump
The Na+ - K+ pump is a complex membrane transport system which catalyzes a simultaneous Na+ i (internal sodium) - K+ o (external potassium) exchange characterized by an asymmetric (3 Na+:2 K+) stoichiometry, responsible for cell polarization. The energy for this active process is supplied by the coupled hydrolisis of ATP. The erythrocyte is the commonest cell model utilized for investigations in this field, mainly because of its easy availability. Furthermore, differently from other cells, Na+ - K+ ATPase from erythrocyte homogenates is exclusively of plasma membrane origin.
KeywordsIntracellular Sodium Extracellular Potassium Pump Unit Ouabain Binding Cation Flux
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