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Evidence of a Positive Membrane Potential and a Calcium Accumulation in Euglena Gracilis Z Mitochondria by in vivo Microelectrode Studies

  • Christian Tamponnet
  • Jean-Pierre Rona
  • Jean-Nöel Barbotin
  • Régis Calvayrac

Abstract

Recently, the biotechnological interest in plant cells has increased considerably and immobilization processes have emerged as an important tool for increasing their longevity (3). The immobilization of Euglena gracilis Z cells in a calcium alginate gel exhibited the conservation of their cellular structures and functions (10). To explain such a behaviour, the role of the high external Ca2+ concentration is suspected. So, the ionic concentrations and mitochondrial membrane potential differences have been measured.

Keywords

Electrochemical Potential Ionic Probe Euglena Gracilis Biotechnological Interest Giant Mitochondrion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. (1).
    Barbier-Brygoo, H., Gibrat, R., Renaudin, J.P., Brown, S., Pradier, J.M., Grignon, C., Guern, J. (1985) Biochim. Biophys. Acta. 819:215–224CrossRefGoogle Scholar
  2. (2).
    Brenneke, R., Lindemann, B. (1971) J. Life Sci. 1:52–58Google Scholar
  3. (3).
    Brodelius, P., Deus, B., Mosbach, K., Zeus, M.H. (1979) FEBS Lett. 103:93–97PubMedCrossRefGoogle Scholar
  4. (4).
    Calvayrac, R., Douce, R. (1970) FEBS Lett. 96:8–17Google Scholar
  5. (5).
    Calvayrac, R., Van Lente, F., Butow, R.A. (1971) Science. 173:252–254PubMedCrossRefGoogle Scholar
  6. (6).
    Campbell, A.K. Intracellular Calcium (Gutfreund ed.) J. Wiley and Sons, New York, 1983Google Scholar
  7. (7).
    Ducet, G. (1984) Physiol. Vég. 22:675–686Google Scholar
  8. (8).
    Maloff, B.L., Scordilis, S.P., Tedeschi, H. (1977) Science. 195:898–900PubMedCrossRefGoogle Scholar
  9. (9).
    Rona, J.P., Van de Sype, G., Cornel, D., Grignon, C., Heller, R. (1980) Bioelectrochem. Bioenerg. 7:377–391CrossRefGoogle Scholar
  10. (10).
    Tamponnet, C., Costantino, F., Barbotin, J.N., Calvayrac, R. (1985) Physiol. Plant. 63:277–283CrossRefGoogle Scholar
  11. (11).
    Tupper, J.T., Tedeschi, H. (1969) Proc. Natl. Acad. Sci. USA. 63:370–377PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Christian Tamponnet
    • 1
    • 2
  • Jean-Pierre Rona
    • 3
  • Jean-Nöel Barbotin
    • 1
  • Régis Calvayrac
    • 2
  1. 1.Lab. de Technologie Enzymatique, UA CNRS 523Université de Technologie de CompiègneCompiègneFrance
  2. 2.Lab. des Membranes Biologiques, Tour 54–53,2°étageUniversité Paris VIIParis Cedex 05France
  3. 3.Lab. d’Electrophysiologie des Membranes, UA CNRS 1180 Tour 54–64, 4°étageUniversité Paris VIIParis Cedex 05France

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