Purification of Jerusalem Artichoke Mitochondria on a Continuous Percoll Gradient

  • Ian M. Møller
  • Annika Lidén

Abstract

Mitochondria from a number of plant species, mainly from green leaves, have been purified on continuous Percoll gradients. In non-green tissues the main contaminants are etioplasts and microbodies and they are removed almost completely through this purification step (e.g. 1). Since we wished to investigate whether there is a homogeneous or a heterogeneous lateral distribution of proteins in the inner mitochondrial membrane (2), it was essential to start with uncontaminated mitochondria. We have, therefore, optimized Percoll purification for Jerusalem artichoke mitochondria.

Keywords

Sucrose Respiration Polypeptide NADH Catalase 

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References

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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Ian M. Møller
    • 1
  • Annika Lidén
    • 1
  1. 1.Department of Plant PhysiologyUniversity of LundLundSweden

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