Purification of Jerusalem Artichoke Mitochondria on a Continuous Percoll Gradient
Mitochondria from a number of plant species, mainly from green leaves, have been purified on continuous Percoll gradients. In non-green tissues the main contaminants are etioplasts and microbodies and they are removed almost completely through this purification step (e.g. 1). Since we wished to investigate whether there is a homogeneous or a heterogeneous lateral distribution of proteins in the inner mitochondrial membrane (2), it was essential to start with uncontaminated mitochondria. We have, therefore, optimized Percoll purification for Jerusalem artichoke mitochondria.
KeywordsSucrose Respiration Polypeptide NADH Catalase
Unable to display preview. Download preview PDF.
- 2.A. Lidén, M. Sommarin, and I.M. Møller, Lateral hecerogeneity in the inner mitochondrial membrane, These proceedings.Google Scholar
- 3.I.M. Møller, I. Ericson, and P. Gardeström, Isolation of submitochondrial particles with different polarities, Methods Enzymol. (In press).Google Scholar
- 7.J.-P. Schwitzguébel, I.M. Møller, and J.M. Palmer, Changes in density of mitochondria and glyoxysomes from Neurospora crassa: a re-evaluation utilizing silica sol gradient centrifugation, J. Gen. Microbiol. 126:289 (1981).Google Scholar