Purification of Jerusalem Artichoke Mitochondria on a Continuous Percoll Gradient

  • Ian M. Møller
  • Annika Lidén


Mitochondria from a number of plant species, mainly from green leaves, have been purified on continuous Percoll gradients. In non-green tissues the main contaminants are etioplasts and microbodies and they are removed almost completely through this purification step (e.g. 1). Since we wished to investigate whether there is a homogeneous or a heterogeneous lateral distribution of proteins in the inner mitochondrial membrane (2), it was essential to start with uncontaminated mitochondria. We have, therefore, optimized Percoll purification for Jerusalem artichoke mitochondria.


Neurospora Crassa Jerusalem Artichoke Plant Mitochondrion Jerusalem Artichoke Tuber Helianthus Tuberosus 
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  1. 1.
    M. Neuburger, E.-P. Journet, R. Bligny, J.-P. Carde, and R. Douce, Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll, Arch. Biochem. Biophys. 217:312 (1982).PubMedCrossRefGoogle Scholar
  2. 2.
    A. Lidén, M. Sommarin, and I.M. Møller, Lateral hecerogeneity in the inner mitochondrial membrane, These proceedings.Google Scholar
  3. 3.
    I.M. Møller, I. Ericson, and P. Gardeström, Isolation of submitochondrial particles with different polarities, Methods Enzymol. (In press).Google Scholar
  4. 4.
    J.M. Palmer, J.-P. Schwitzguébel, and I.M. Møller, Regulation of malate oxidation in plant mitochondria. Response to rotenone and exogenous NAD+, Biochem. J. 208:703 (1982).PubMedGoogle Scholar
  5. 5.
    D.A. Day, M. Neuburger, and R. Douce, Biochemical characterization of chlorophyll-free mitochondria from pea leaves, Aust. J. Plant Physiol. 12:219 (1985).CrossRefGoogle Scholar
  6. 6.
    A. Tobin, B. Djerdjour, E. Journet, M. Neuburger, and R. Douce, Effect of NAD+ on malate oxidation in intact plant mitochondria, Plant Physiol. 66:225 (1980).PubMedCrossRefGoogle Scholar
  7. 7.
    J.-P. Schwitzguébel, I.M. Møller, and J.M. Palmer, Changes in density of mitochondria and glyoxysomes from Neurospora crassa: a re-evaluation utilizing silica sol gradient centrifugation, J. Gen. Microbiol. 126:289 (1981).Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Ian M. Møller
    • 1
  • Annika Lidén
    • 1
  1. 1.Department of Plant PhysiologyUniversity of LundLundSweden

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