Active Transport of Adenosine into Primary Cultures of Brain Cells and its Methodological Consequences

  • Leif Hertz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 309A)


In spite of the ability of most mammalian cells to synthesize nucleosides, the presence of transport mechanisms for these compounds across cell membranes is well established. Their presence is of importance for several reasons: 1) some cells, e. g., erythrocytes, leucocytes, bone marrow cells, cells in the gastrointestinal tract and perhaps some brain cells are deficient in de novo synthesis of purines or pyrimidines and thus need to accumulate purines or pyrimidines which have either been ingested or produced in other cells; 2) the physiological role of certain cells, e. g., in the kidney or in the choroid plexes, is to transport different compounds (which may include nucleosides) across a barrier between different compartments, and 3) some cells, predominantly in the central nervous and cardiovascular systems, express receptors for nucleosides on their cell surface, and cellular uptake is an important mechanism to terminate the action of many transmitters.


Active Uptake Nucleoside Transport Adenosine Concentration Active Transport Mechanism Diffusional Uptake 
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  1. Geiger, J.D., Johnston, M.E., and Yago, V., Pharmacological characterization of rapidly accumulated adenosine by dissociated brain cells from adult rat, J. Neurochem., 51, 283–291, 1988.PubMedCrossRefGoogle Scholar
  2. Hertz, L. Nucleoside transport in cells: kinetics and inhibitor effects. In Adenosine and the Adenine Nucleotides as Regulators of Cellular Function (J.W. Phillis, Ed.), pp. 85-107, CRC Press, Boca Raton, 1991.Google Scholar
  3. Hertz, L., Juurlink, B.H.J., and Szuchet, S. Cell cultures. In Handbook of Neurochemistry, (A. Lajtha, Ed.), 2nd Ed., Vol. 8. Plenum Press, New York, pp. 603–661, 1985.Google Scholar
  4. Matz, H., and Hertz, L., Effects of an adenosine deaminase inhibitor on active uptake and metabolism of adenosine in astrocytes in primary cultures, Brain Res. 515, 168–172, 1990.PubMedCrossRefGoogle Scholar
  5. Johnston, M.E., and Geiger, J.D., Sodium-dependent uptake of nucleosides by dissociated brain cells from the rat, J. Neurochem., 52, 75–81, 1989.PubMedCrossRefGoogle Scholar
  6. Paterson, A.R.P., Kolassa, N., and Cass, C.E., Transport of nucleoside drugs in animal cells, Pharmac. Ther., 12, 515–536, 1981.CrossRefGoogle Scholar
  7. Thampy, K.G., and Barnes, E.M. Jr., Adenosine transport by cultured glial cells from chick embryo brain, Arch. Biochem. Biophys., 220, 340–346, 1983.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Leif Hertz
    • 1
  1. 1.Depts. of Pharmacology and AnaesthesiaUniversity of SaskatchewanSaskatoonCanada

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