Active Transport of Adenosine into Primary Cultures of Brain Cells and its Methodological Consequences
In spite of the ability of most mammalian cells to synthesize nucleosides, the presence of transport mechanisms for these compounds across cell membranes is well established. Their presence is of importance for several reasons: 1) some cells, e. g., erythrocytes, leucocytes, bone marrow cells, cells in the gastrointestinal tract and perhaps some brain cells are deficient in de novo synthesis of purines or pyrimidines and thus need to accumulate purines or pyrimidines which have either been ingested or produced in other cells; 2) the physiological role of certain cells, e. g., in the kidney or in the choroid plexes, is to transport different compounds (which may include nucleosides) across a barrier between different compartments, and 3) some cells, predominantly in the central nervous and cardiovascular systems, express receptors for nucleosides on their cell surface, and cellular uptake is an important mechanism to terminate the action of many transmitters.
KeywordsActive Uptake Nucleoside Transport Adenosine Concentration Active Transport Mechanism Diffusional Uptake
Unable to display preview. Download preview PDF.
- Hertz, L. Nucleoside transport in cells: kinetics and inhibitor effects. In Adenosine and the Adenine Nucleotides as Regulators of Cellular Function (J.W. Phillis, Ed.), pp. 85-107, CRC Press, Boca Raton, 1991.Google Scholar
- Hertz, L., Juurlink, B.H.J., and Szuchet, S. Cell cultures. In Handbook of Neurochemistry, (A. Lajtha, Ed.), 2nd Ed., Vol. 8. Plenum Press, New York, pp. 603–661, 1985.Google Scholar