Cellular Spin Resonance

  • Ingolf Lamprecht
  • Maja Mischel

Abstract

Among the different effects living cells exhibit under the influence of external electric fields, rotation of cells is one of the most predominant ones. Teixeira-Pinto et al. (1960) were supposedly the first to report about the spinning of parts of amoebae in a high-frequency field; Füredi and Ohad (1964) observed rotation of erythrocytes and Pohl and Crane (1971) of yeasts. It became clear in the following years that rotation in external fields is a general phenomenon of cells, live as well as dead ones, and that the way of spinning could provide information on the biological and physiological state of the cell under investigation. As spinning often started at a very sharp frequency of the applied field and ceased in the same manner, Pohl and Crane (1971) coined the descriptive name “cellular spin resonance” (CSR) which will be used throughout this chapter.

Keywords

Sucrose Glycerol Agar Torque Platinum 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arno, R., 1892, Campo elettrico rotante e rotazioni dovute all’isteresi elettrostatica, Atti Accad. Lincei Rend. Roma I(5):284–289.Google Scholar
  2. Arnold, W. M., and Zimmermann, U., 1982, Rotating-field-induced rotation and measurement of the membrane capacitance of single mesophyll cells of Avena sativa, Z. Naturforsch. 37c:908–915.Google Scholar
  3. Beezer, A. E., and Sharma, P. B., 1981, On the uptake of nystatin by Saccharomyces cerevisiae. 3. Electrochemistry of the yeast cell surface, Microbios 31:71–82.PubMedGoogle Scholar
  4. Born, M., 1920, Über die Beweglichkeit der elektrolytischen Ionen, Z. Phys. 1:221–249.CrossRefGoogle Scholar
  5. Fröhlich, H., 1968, Long-range coherence and energy storage in biological systems, Int. J. Quant. Chem. 2:641–649.CrossRefGoogle Scholar
  6. Fuhr, G., 1985, Über die Rotation dielektrischer Körper in rotierenden Feldern, Dissertation, Humboldt-Universität, Berlin.Google Scholar
  7. Fuhr, G., Hagedorn, R., and Göring, H., 1984, Cell rotation in a discontinuous field of a 4-electrode chamber, Stud. Biophys. 102:221–227.Google Scholar
  8. Fuhr, G., Hagedorn, R., and Göring, H., 1985a, Separation of different cell types by rotating electric fields, Plant Cell Physiol. 26:1527–1531.Google Scholar
  9. Fuhr, G., Hagedorn, R., and Müller, T., 1985b, Simulation of the rotational behaviour of single cells by macroscopic spheres, Stud. Biophys. 107:109–116.Google Scholar
  10. Füredi, A. A., and Ohad, I., 1964, Effects of high-frequency electric fields on the living cell, Biochim. Biophys. Acta 79:1–8.Google Scholar
  11. Glaser, R., Fuhr, G., and Gimsa, J., 1983, Rotation of erythrocytes, plant cells and protoplasts in an outside rotating electric field, Stud. Biophys. 96:11–20.Google Scholar
  12. Hertz, H. R., 1880, Inaugural-Dissertation, in: Gesammelte Werke von Heinrich Hertz, Volume 1 (J. A. Barth, ed.), Leipzig, 1895.Google Scholar
  13. Hertz, H. R., 1881, IV. Über die Verteilung der Electrizität auf der Oberfläche bewegter Leiter, Ann. Phys. Chem. 13:266–275.Google Scholar
  14. Hölzel, R., 1988, Sine quadrature oscillator for cellular spin resonance up to 120 MHz, Med. Biol. Eng. Comput. 26:102–105.CrossRefGoogle Scholar
  15. Hölzel, R., and Lamprecht, I., 1987, Cellular spin resonance of yeast in a frequency range up to 140 MHz, Z. Naturforsch. 42c: 1367–1369.Google Scholar
  16. Holzapfel, C., Vienken, J., and Zimmermann, U., 1982, Rotation of cells in an alternating electric field: Theory and experimental proof, J. Membr. Biol. 67:13–26.PubMedCrossRefGoogle Scholar
  17. James, A. M., 1979, Electrophoresis of particles in suspension, Surf. Colloid Sci. 11:121–185.CrossRefGoogle Scholar
  18. Lampa, A., 1906, Über Rotationen im elektrostatischen Drehfelde, Sitzungsber. Akad. Wiss. Wien 115(IIa): 1659–1690.Google Scholar
  19. Lertes, P., 1921a, Untersuchungen über Rotationen von dielektrischen Flüssigkeiten im elektrostatischen Drehfeld, Z. Phys. 4(3):315–336.CrossRefGoogle Scholar
  20. Lertes, P., 1921b, Der Dipolrotationseffekt bei dielektrischen Flüssigkeiten, Z. Phys. 6:56–68.CrossRefGoogle Scholar
  21. Lopez, M. C., Iglesias, F. J., Santamaria, C., and Dominguez, A., 1984, Dielectrophoretic behaviour of Saccharomyces cerevisiae: Effect of cations and detergents, FEMS Microbiol. Lett. 24:149–152.CrossRefGoogle Scholar
  22. Mischel, M., and Lamprecht, I., 1980, Dielectrophoretic rotation in budding yeast cells, Z. Naturforsch. 35c: 1111–1113.Google Scholar
  23. Mischel, M., and Lamprecht, I., 1983, Rotation of cells in nonuniform fields, J. Biol. Phys. 11:43–44.CrossRefGoogle Scholar
  24. Mischel, M., and Pohl, H. A., 1983, Cellular spin resonance: Theory and experiment, J. Biol. Phys. 11:98–102.CrossRefGoogle Scholar
  25. Mischel, M., Voss, A., and Pohl, H. A., 1982, Cellular spin resonance in rotating electric fields, J. Biol. Phys. 10:223–226.CrossRefGoogle Scholar
  26. Phillips, W., Pohl, H. A., and Pollock, J. K., 1985, Evidence for AC fields produced by mammalian cells, Proc. 1985 Annu. Conf. Institute of Electrostatics, Japan.Google Scholar
  27. Pilwat, G., and Zimmermann, U., 1983, Rotation of a single cell in a discontinuous rotating electric field, Bioelectrochem. Bioenerg. 10:155–162.CrossRefGoogle Scholar
  28. Pohl, H. A., 1978, Dielectrophoresis—The Behavior of Neutral Matter in Nonuniform Electric Fields, Cambridge University Press, London.Google Scholar
  29. Pohl, H. A., 1981a, Cellular spin resonance, J. Theor. Biol. 93:207–213.CrossRefGoogle Scholar
  30. Pohl, H. A., 1981b, Natural electrical rf oscillation from cells, J. Bioenerg. Biomembr. 13:149–169.PubMedCrossRefGoogle Scholar
  31. Pohl, H. A., 1982, Natural cellular electrical resonances, Int. J. Quantum Chem. 9:399–409.Google Scholar
  32. Pohl, H. A., 1983a, Cellular spinning in pulsed rotating electric fields, J. Biol. Phys. 11:59–62.CrossRefGoogle Scholar
  33. Pohl, H. A., 1983b, The spinning of suspended particles in a two-pulsed, three-electrode system, J. Biol. Phys. 11:66–68.CrossRefGoogle Scholar
  34. Pohl, H. A., and Braden, T., 1982, Cellular spin resonance of aging yeast and mouse sarcoma cells, J. Biol. Phys. 10:17–30.CrossRefGoogle Scholar
  35. Pohl, H. A., and Crane, J. S., 1971, Dielectrophoresis of cells, Biophys. J. 11:711–727.PubMedCrossRefGoogle Scholar
  36. Quincke, G., 1896, Über Rotationen im constanten electrischen Felde, Ann. Phys. Chem. 59:417–486.Google Scholar
  37. Quincke, G., 1897, Die Klebrigkeit isolierender Flüssigkeiten im constanten electrischen Felde, Ann. Phys. Chem. 62:1–13.Google Scholar
  38. Rivera, H., and Pohl, H. A., 1986, Cellular spin resonance (CSR), in: Modern Bioelectrochemistry (F. Gutmann and H. Keyzer, eds.), Plenum Press, New York, pp. 431–444.CrossRefGoogle Scholar
  39. Sauer, F. A., and Schlögl, R. W., 1985, Torques exerted on cylinders and spheres by external electromagnetic fields: A contribution to the theory of field induced cell rotation, in: Interactions between Electromagnetic Fields and Cells (A. Chiabrera, C. Nicolini, and H. P. Schwan, eds.), Plenum Press, New York, pp. 203–251.Google Scholar
  40. Teixeira-Pinto, A. A., Nejelski, L. L., Jr., Cutler, J. L., and Heller, J. H., 1960, The behavior of unicellular organisms in an electromagnetic field, Exp. Cell Res. 20:548–564.CrossRefGoogle Scholar
  41. von Schweidler, E. R., 1897, Über Rotationen im homogenen elektrischen Felde, Sitzungsber. Akad. Wiss. Wien 106(IIa):526–532.Google Scholar
  42. Wicher, D., Gündel, J., and Matthies, H., 1986, Measuring chamber with extended applications of the electrorotation. α-and β-dispersion of lipsomes, Stud. Biophys. 115:51–58.Google Scholar
  43. Zimmermann, U., Vienken, J., and Pilwat, G., 1981, Rotation of cells in an alternating electric field: The occurrence of a resonance frequency, Z. Naturforsch. 36c: 173–177.Google Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Ingolf Lamprecht
    • 1
  • Maja Mischel
    • 1
  1. 1.Institute for BiophysicsFree University of BerlinBerlin 33Federal Republic of Germany

Personalised recommendations