Colour Vision and Immunologically Identifiable Photoreceptor Subtypes

  • Àgoston Szèl
  • Pal Röhlich

Abstract

Colour vision is based on a family of visual pigments localized in the retinal photoreceptor cells. Monoclonal and polyclonal antibodies generated against these proteins were used to discriminate the rods and the cone types of various vertebrate species. The different cell types were demonstrated by light microscopic immunocytochemistry on retinal sections of the turtle, the gecko, and several mammalian species. The human retina was also included. A good correlation could be found between the colour-sensitivities and the immunologic staining pattern of the photoreceptor subpopulations. This novel approach to distinguish the visual cells with immunocytochemistry proved to be useful to map the distribution of the colour-specific elements of the retina.

Keywords

Albumin Hexagonal Retina Dehydration Glutaraldehyde 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ahnelt, P. K., 1974, Characterization of the color related receptor mosaic in the ground squirrel retina, Vision Res., 25: 1557–1567.CrossRefGoogle Scholar
  2. Bowmaker, J. K., 1977, The visual pigments, oil droplets and spectral sensitivity of the pigeon, Vision Res., 17: 1129–1138.PubMedCrossRefGoogle Scholar
  3. Bowmaker, J. K., Knowles, A., 1977, The visual pigments and oil droplets of the chicken retina, Vision Res., 17: 755–764.PubMedCrossRefGoogle Scholar
  4. Caldwell, J. H., Daw, N. W., 1978, New properties of rabbit retinal ganglion cells, J. Physiol. 216: 216–251.Google Scholar
  5. Cserhàti, P., Szél, À., Röhlich, P., 1988, Four cone types characterized by anti-visual pigment antibodies in the red area of the pigeon retina, Invest Ophthalmol. Vis. Sci., In PressGoogle Scholar
  6. Daw, N. W., Pearlman, A. L., 1970, Cat colour vision: evidence for more than one cone process, J. Physiol., 211: 125–137.PubMedGoogle Scholar
  7. De Monasterio, F. M., Schein, S. J., McCrane, E. P., 1981, Staining of blue-sensitive cones of the macaque retina by a fluorescent dye, Science 213: 1278–1281.CrossRefGoogle Scholar
  8. Gaur, V. P., Adamus, G., Arendt, A., Eldred, W., Possin, D. E., McDowell, J. H., Hargrave, P. A., Sarthy, P. V., 1988, A monoclonal antibody that binds to photoreceptors in the turtle retina, Vision Res., 28: 765–776PubMedCrossRefGoogle Scholar
  9. Granda, A. M., Dvorak, C. A., 1977, Vision in turtles, Photoreceptors, in: “The Visual System in Vertebrates. Handbook of Sensory Physiology, Vol. VII/5,” Crescitelli, F. ed., Springer Verlag, Berlin, Heidelberg, New York.Google Scholar
  10. Kolb, H., Jones, J., 1987, The distinction by light and electron microscopy of two types of cone containing colorless oil droplets in the retina of the turtle, Vision Res., 27: 1445–1458PubMedCrossRefGoogle Scholar
  11. Liebman, P., 1972, Microspectrophotometry of photoreceptors, in: “The Handbook of Sensory Physiology VII/1,” Dartnal, H.J.A. ed., Springer, Berlin.Google Scholar
  12. Liebman, P., Granda, A. M., 1971, Microspectrophotometric measurements of visual pigments in two species of turtle, Pseudemys scripta and Chelonia mydas, Vision Res., 11: 105–114.PubMedCrossRefGoogle Scholar
  13. Mansfield, R. J. W., Levine, J. S., Lipetz, L. E., Collins, B. A., Raymond, G., MacNichol, E. F., 1984, Blue-sensitive cones in the primate retina: microspectrophotometry of the visual pigment, Exp. Brain. Res., 56: 389–394.PubMedCrossRefGoogle Scholar
  14. Marc, R. E., Sperling, H. G., 1977, Chromatic organization of primate cones, Science, 196: 454–456.PubMedCrossRefGoogle Scholar
  15. Mayor, H. D., Hampton, J. C., Rosario, B., 1961, A simple method for removing the resin from the epoxy-embedded tissue, J. Biophys. Biochem. Cytol., 9: 909–910.PubMedCrossRefGoogle Scholar
  16. Nathans, J., Hogness, D., Hogness, D. S., 1986, Molecular genetics of human color vision: the genes encoding blue, green, and red pigments, Science, 232: 193–202.PubMedCrossRefGoogle Scholar
  17. Nuboer, J. F. W., 1986, A comparative view on colour vision, Netherlands J. Zool., 36: 344–380.CrossRefGoogle Scholar
  18. Nunn, B. J., Schnapf, J. L., Baylor, D. A., 1984, Spectral sensitivity of single cones in the retina of Macaca fascicularis, Nature, 309: 264–266.PubMedCrossRefGoogle Scholar
  19. Ohtsuka, T., 1985, Relation of spectral types to oil droplets in cones of turtle retina, Science, 229: 874–877.PubMedCrossRefGoogle Scholar
  20. Schnapf, J. L., Kraft, T. W., Baylor, D. A., 1987, Spectral sensitivity of human cone photoreceptors, Nature, 325: 439–441.PubMedCrossRefGoogle Scholar
  21. Sperling, H. G., Johnson, C., Harwerth, R. S., 1980, Differential spectral photic damage to primate cones, Vision Res., 20: 1117–1125.PubMedCrossRefGoogle Scholar
  22. Szél, À., Takàcs, L., Monostori, E., Vigh-Teichmann, I., Röhlich, P., 1985, Heterogeneity of chicken photoreceptors as defined by hybridoma supernatnats, Cell Tiss. Res., 240: 741–755.Google Scholar
  23. Szél, À., Röhlich, P., Govardovskii, V., 1986a, Moonoclonal antibody recognizing cone visual pigment, Exp. Eve Res., 43: 871–883.CrossRefGoogle Scholar
  24. Szél, À, Takàcs, L., Monostori, É., Diamantstein, T., Vigh-Teichmann, I., Röhlich, P., 1986a, Monoclonal antibody recognizing cone visual pigment, Exp. Eye Res., 43: 871–883.PubMedCrossRefGoogle Scholar
  25. Szél, À., Röhlich, P., Govardovskii, V., 1986b, Immunocytochemical discrimination of visual pigments in the retinal photoreceptors of the nocturnal gecko Teratoscincus scincus, Exp. Eye Res., 43: 895–904.PubMedCrossRefGoogle Scholar
  26. Szél, AÀ., Diamantsein, T., Röhlich, P., 1988, Identification of the blue-sensitive cones in the mammalian retina by anti-visual pigment antibody, J. Comp. Neurol., 273: 593–602.PubMedCrossRefGoogle Scholar
  27. Szél, À., Röhlich, P., 1988a, Four photoreceptor types in the ground squirrel retina as evidenced by immunocytochemistry, Vision Res., In Press.Google Scholar
  28. Szél, À., Röhlich, P., 1988b, The mosaic of the colour-specific photoreceptors in the mammalian retina as defined by immunocytochemistry, Acta. Morph., In press.Google Scholar
  29. Van Veen, T., Vigh-Teichmann, L, Vigh, B., Hartwig, H. G., 1986, Light and electron microscopy of S—antigen-and opsin-immunoreactive photoreceptors in the retina of turtle, chicken, and hedgehog, Exp. Biol., 45: 1–14.PubMedGoogle Scholar
  30. Wald, G., Brown, P. K., Smith, P. H., 1953, Cyanopsin, a new pigment of cone vision, Science, 118: 505–508.PubMedCrossRefGoogle Scholar
  31. Wald, G., Brown, P. K., Smith, P. H., 1955, Iodopsin, J. Gen. Physiol., 38: 623–681.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Àgoston Szèl
    • 2
  • Pal Röhlich
    • 1
  1. 1.I. Laboratory of Electron MicroscopySemmelweis University of MedicineBudapestHungary
  2. 2.2nd Department of Anatomy, Histology and EmbryologySemmelweis University of MedicineBudapestHungary

Personalised recommendations