The Cut-Open Axon Technique

  • Francisco Bezanilla
  • Carol Vandenberg

Abstract

The giant axon of the squid has been the preparation of choice for the recording of electrical events associated with the opening and closing of the ionic conductances. Its large size allows the introduction of electrodes and the exchange of solutions giving almost complete control of the chemical environment and voltage across the axolemma (Adelman and Gilbert, 1990; Mullins and Brinley, 1990).

Keywords

Sucrose Ethyl HEPES Prep EGTA 

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References

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Francisco Bezanilla
    • 1
  • Carol Vandenberg
    • 2
  1. 1.Department of Physiology, Ahmanson Laboratory of Neurobiology and Jerry Lewis Neuromuscular CenterUniversity of California at Los AngelesLos AngelesUSA
  2. 2.Department of Biological SciencesUniversity of California at Santa BarbaraSanta BarbaraUSA

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