The Cut-Open Axon Technique
The giant axon of the squid has been the preparation of choice for the recording of electrical events associated with the opening and closing of the ionic conductances. Its large size allows the introduction of electrodes and the exchange of solutions giving almost complete control of the chemical environment and voltage across the axolemma (Adelman and Gilbert, 1990; Mullins and Brinley, 1990).
KeywordsSucrose Ethyl HEPES Prep EGTA
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