Spatiotemporal Inhomogeneity of [Ca]i in Neurons

  • S. L. Mironov
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 333)


The concept that Ca might serve as an intracellular messenger to couple stimulus to response in excitable tissues is well established. An understanding of the precise mechanisms by which second messengers act, requires information on the temporal nature of changes in second messenger concentration and their spatial distibution within the cell. By optical imaging with fluorescent Ca-indicators, it was found that the change in intracellular free Ca-concentration is often transient and spatially inhomogeneous (for a recent review see Tsien and Tsien, 1990). Appreciation of this particular paradox leads to the recognition that Ca-cycling in the target cell has an important, spatially and temporally distinct messenger function. The purpose of this communication is to consider the intracellular messenger role of Ca in generation of specific transients and gradients in the cytoplasm of nerve cells.


Membrane Potential Oscillation Bovine Adrenal Chromaffine Cell Single Exponent Digital Fluorescence Microscopy Messenger Concentration 
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  1. Bezprozvanny I. Watras J. and Ehrlich B. E. (1991) Bell-shaped Ca-response curves of IP3-and Ca-gated channels from endoplasmic reticulum of cerebellum. Nature 351: 751–753.PubMedCrossRefGoogle Scholar
  2. Fabiato A. (1983) Ca-induced release of Ca from the cardiac sarcoplasmic reticulum. Amer. J. Physiol. 245: 1–14.Google Scholar
  3. Finch E. A. Turner T. J. and Goldin S. M. (1991) Ca as a coagonist of IP3-induced Ca release. Science 252: 443–445.PubMedCrossRefGoogle Scholar
  4. Gorman A. and Thomas M. (1978) Changes in the intracellular Ca concentration in a pacemaker neuron. J. Physiol. 275: 357–373.PubMedGoogle Scholar
  5. Hodgkin A. and Huxley A. (1952) A quantitative description of membrane current and its application to conduction and excitation in nerve. J. Physiol. 117: 500–542.PubMedGoogle Scholar
  6. Kirischuk S. I. Zhukarev V. A. and Mironov S. L. (1990) 2D-analysis of biological microobject images by means of digital fluorescence microscopy. Biol. memb. 7: 399–405.Google Scholar
  7. Neher E. (1986) Concentration profile of intracellular Ca in the presence of diffusible chelators. Exp. Brain Res. S14: 80–96.Google Scholar
  8. Kostyuk P. Mironov S. Tupikin A. and Belan P. (1990) Cytoplasmic free [Ca]i in isolated snail neurones as revealed by fura-2. J. Membrane Biol. 110: 11–20.CrossRefGoogle Scholar
  9. Lipscombe D. Madison D. Poenie M. Reuter H. Tsien R.Y. and Tsien R. W. (1988) Spatial distribution of Ca channels and cytosolic Ca transients in growth cones and cell bodies of sympathetic neurones. Proc. Natl. Acad.Sci. USA 85: 2398–2402.PubMedCrossRefGoogle Scholar
  10. Meech R. (1979) Membrane potential oscillations in molluscan bursting neurones. J. Exp. Biol. 81: 93–115.PubMedGoogle Scholar
  11. Miller R. J. (1991) The control of neuronal Ca homeostasis. Progr. Neurobiol. 37: 255.CrossRefGoogle Scholar
  12. Mironov S. L. (1983) Slow wave membrane potential oscillations in mollusc neurones. Neuroscience 10: 899–907.PubMedCrossRefGoogle Scholar
  13. Mironov S. L. and Tupikin A. V. (1988) On the nature of periodic changes in the intracellular Ca concentration. Biol. Memb. 5: 528–534.Google Scholar
  14. Mironov S. L. (1990) Theoretical analysis of propagation of [Ca]i wave along the surface of intracellular stores. J.Theor. Biol. 146: 87–98.PubMedCrossRefGoogle Scholar
  15. Mironov S. L. and Usachev J. M. (1990) Sr and Ba transients in isolated snail neurones studied with fura-2. Neurosci. Lett. 112: 184–189.PubMedCrossRefGoogle Scholar
  16. Mironov S. L. and Usachev J. M. (1991) Caffeine affects Ca uptake and Ca release from intracellular stores. Neurosci.Lett. 123: 200–204.PubMedCrossRefGoogle Scholar
  17. O’Sullivan A. Check T. Moreton R. Berridge M. and Burgoyne R. (1989) Localization and heterogeneity of agonist-induced changes in cytosolic Ca concentration in single bovine adrenal chromaffine cells. EMBO J. 8: 401–411.PubMedGoogle Scholar
  18. Ross W. N. Stockbridge L. L. and Stockbridge N. L. (1986) Regional properties of Ca entry in barnacle neurons determined with Arsenazo III and a photodiode array. J.Neurosci. 6: 1148–1156.PubMedGoogle Scholar
  19. Tsien R. W. and Tsien R. Y. (1990) Ca channels stores and oscillations. Ann. Rev. Cell. Biol. 6: 715–745.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • S. L. Mironov
    • 1
  1. 1.Department of NeurophysiologyMax-Planck Institute for PsychiatryPlanegg-MartinsriedGermany

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