Heparan Sulphate Proteoglycans: Molecular Organisation of Membrane-Associated Species and an Approach to Polysaccharide Sequence Analysis
Heparan sulphate proteoglycans (HSPGs) are normally the most abundant PG components of cell surfaces and basement membranes (1, 2). At the molecular level five core proteins have been described that can be glycanated with HS chains (2, 3) but evidence exists for several other probably distinct species including two cell surface HSPGs in which the core proteins have been shown to bind either TGF-ß (4) or fibroblast growth factor (FGF;5); the former has been named betaglycan. A recent surprising finding was that the secretory granule PG, named serglycin, which normally appears as a heparin or chondroitin sulphate PG, is synthesised as an HSPG in an erythroid cell line (6). The topographical distribution of HSPGs is therefore variable and extensive and probably reflects a broad functional spectrum to which both protein and polysaccharide components will make significant contributions (1, 2, 7).
KeywordsHeparin Polysaccharide Xylose Oligosaccharide Mast
Unable to display preview. Download preview PDF.
- 6.Okayama, M., Oguri, K., Yoshida, K., and Ohkita, T. (1991). Purification and characterisation of novel heparan sulphate proteoglycans produced by murine erythroleukaemia cells in the growing phase. J. Biol. Chem. 266: 38080.Google Scholar
- 12.Pierce, A., Cowling, G., Lyon, M. and Gallagher, J.T.-unpublished observations.Google Scholar
- 15.Ishihara, M., Fedarko, N. and Conrad, H.E. (1983). Involvement of phosphatidylinositol and insulin in the coordinate regulation of proteoheparan sulphate metabolism and hepatocyte growth. J. Biol. Chem. 262: 4708.Google Scholar
- 17.Yanagishita, M. and McQuillan, D.J. (1989). Two forms of plasma membrane — intercalated heparan sulphate proteoglycan in rat ovarian granulosa cells. Labelling of proteoglycans with a photoactivatable hydrophobic probe and effect of the membrane anchor-specific phospholipase C. J. Biol. Chem. 264: 17551.PubMedGoogle Scholar
- 24.Gallagher, J.T., and Lyon, M. (1989). Molecular organisation and functions of heparan sulphate. In Heparin, ed by Lane, D.A. and Lindahl, U. pp 135 Edward Arnold, London.Google Scholar
- 27.Lindahl, U. (1989). Biosynthesis of heparin and related polysaccharides. In: Heparin, ed. by Lane D.A., and Lindahl, U. pp 159, Edward Arnold, London.Google Scholar
- 31.Gallagher, J.T., Turnbull, J.E. and Lyon, M. (1991). Patterns of sulphation in heparan sulphate: polymorphism based on a common structural theme. Int. J. Biochem. — in press.Google Scholar
- 32.Turnbull, J.E. (1991). Oligosaccharide mapping and sequence analysis of glycosaminoglycans. In: Methods in Molecular Biology — Membrane Methods. Humana Press, New Jersey — in press.Google Scholar