Heparan Sulphate Proteoglycans: Molecular Organisation of Membrane-Associated Species and an Approach to Polysaccharide Sequence Analysis
Heparan sulphate proteoglycans (HSPGs) are normally the most abundant PG components of cell surfaces and basement membranes (1, 2). At the molecular level five core proteins have been described that can be glycanated with HS chains (2, 3) but evidence exists for several other probably distinct species including two cell surface HSPGs in which the core proteins have been shown to bind either TGF-ß (4) or fibroblast growth factor (FGF;5); the former has been named betaglycan. A recent surprising finding was that the secretory granule PG, named serglycin, which normally appears as a heparin or chondroitin sulphate PG, is synthesised as an HSPG in an erythroid cell line (6). The topographical distribution of HSPGs is therefore variable and extensive and probably reflects a broad functional spectrum to which both protein and polysaccharide components will make significant contributions (1, 2, 7).
KeywordsHeparan Sulphate Core Protein Heparan Sulphate Proteoglycan Human Lung Fibroblast Mouse Mammary Epithelial Cell
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