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Branched Polypeptides with a Poly(L-Lysine) Backbone: Synthesis, Conformation, and Immunomodulation

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Polymeric Materials in Medication

Part of the book series: Polymer Science and Technology ((POLS,volume 32))

Abstract

We have developed a group of branched (multichain) poly-peptides composed of a poly(L-lysine) backbone with short oligomeric poly(DL-alanine) side chains (the inside area) and one, two or more other amino acid residues (the outside determinant). These polypeptides were characterized by amino acid analysis, identification of terminal amino acids of the side chains, sedimentation analysis, and thin-layer and column gel chromatography. The conformation of the polypeptides was analyzed by circular dichroism spectroscopy of water-alcohol mixtures and of water solutions at various pH and ionic strengths. These data indicated a marked dependence of the conformation on the identity, charge and absolute configuration of the side chain terminal amino acids and on the number of these residues present. The immunomodulatory potential of one of these polypeptides was investigated. Dose dependence, immunization schedule and ability of this polypep-tide to compensate for the immunosuppressive effect of cyto-toxic drugs were evaluated.

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References

  1. M. Przybylsi, D. S. Zaharko, M. A. Chirigos, R. H. Adamson, R. M. Schultz, and H. Rinsdorf, Cancer Treatment Reports 62, 1837 (1978).

    Google Scholar 

  2. M. Szekerke, R. Wade, M. E. Whisson, Neoplasma, 19, 199 (1972).

    Google Scholar 

  3. M. Szekerke, R. Wade, M. E. Whisson, Neoplasma, 19, 121 (1972).

    Google Scholar 

  4. M. Szekerke, J. S. Driscoll, Eur. J. Cancer, 13, 529 (1977).

    Google Scholar 

  5. B. Benacerraf, D. H. Katz, Adv. Cancer Res., 21, 121 (1975).

    Article  Google Scholar 

  6. M. Sela, in: “Peptides, Polypeptides, Proteins”, E. R. Blout, F. A. Bovey, H. Goodman, N. Zotan, Eds., Wiley, New York, 1974, p 495.

    Google Scholar 

  7. L. Chedid, M. Parant, F. Parant, F. Audibert, F. Lefrancier, J. Choay and M. Sela, Proc. Natl. Acad. Sci., USA, 76, 6557 (1979).

    Article  ADS  Google Scholar 

  8. G. M. Muller, I. M. Shapiro, R. Arnon, Proc. Natl. Acad. Sci., USA, 79, 569 (1982).

    Article  ADS  Google Scholar 

  9. F. Audibert, M. Jolivet, L. Chedid, R. Arnon, and M. Sela, Proc. Natl. Acad. Sci. USA, 79, 5042 (1982).

    Article  ADS  Google Scholar 

  10. F. Hudecz and M. Szekerke, Coll. Czech. Commun., 45, 933 (1980).

    Google Scholar 

  11. F. Hudecz and M. Szekerke, Coll. Czech. Commun., 50, 103 (1985).

    Article  Google Scholar 

  12. N. Anand, N. S. R. K. Murthy, F. Naider, M. Goodman, Macromolecules, 4, 564 (1971).

    Article  ADS  Google Scholar 

  13. L. Michielin, S. Mammi, E. Peggion, Biopolymers, 22, 2325 (1983).

    Article  Google Scholar 

  14. A. Yaron, A. Berger, Biochem. Biophys. Acta, 107, 307 (1965).

    Article  Google Scholar 

  15. H. Votavova, F. Hudecz, J. Kajtar, M. Szekerke, J. Sponar, K. Blaha, Coll. Czech. Chem. Commun., 45, 941 (1980).

    Google Scholar 

  16. H. Votavova, F. Hudecz, J. Sponar, K. Blaha, M. Szekerke, Coll. Czech. Chem. Commun., 47, 3437 (1982).

    Article  Google Scholar 

  17. H. Votavova, F. Hudecz, J. Kajtar, J. Sponar, K. Blaha, M. Szekerke, Coll. Czech. Chem. Commun., 50, 228 (1985).

    Article  Google Scholar 

  18. IUPAC-IUB Commission on Biochemical Nomenclature, J. Biol. Chem., 247, 977 (1972).

    Google Scholar 

  19. IUPAC-IUB Commission on Biochemical Nomenclature, Biochem. J., 127, 753 (1972).

    Google Scholar 

  20. G. Folsch, K. Serck-Hanssen, Acta Chem. Scand., 13, 1243 (1959).

    Article  Google Scholar 

  21. G. D. Fasman, M. Idelson, and E. R. Blout, J. Am. Chem. Soc, 83, 709 (1961).

    Article  Google Scholar 

  22. E. Katchalsky, I. Grossfeld, and M. Frankel, J. Am. Chem. Soc, 70, 2094 (1948).

    Article  Google Scholar 

  23. A. Licht, B. Schecter, M. Sela, Eur. J. Immunol., 1, 351 (1971).

    Article  Google Scholar 

  24. M. Sela, and A. Berger, J. Am. Chem. Soc, 77, 1893 (1955).

    Article  Google Scholar 

  25. E. Wunsch, and A. Zwick, Chem. Ber., 97, 2497 (1964).

    Article  Google Scholar 

  26. H. Yajima, and K. Kubo, J. Am. Chem. Soc, 87, 2039 (1965).

    Article  Google Scholar 

  27. A. B. Zegelman, T. Yu. Yresupov, Sh. Ch. Khalezdov, V. A. Shiber, Khim. Prirod, Soedin, 9, 773 (1973).

    Google Scholar 

  28. J. Kovacs, M. Q. Ceprini, and C. A. Dupraz, G. N. Schmit, J. Org. Chem, 32, 3696 (1967).

    Article  Google Scholar 

  29. E. R. Blout, R. H. Karlsen, P. Doty, B. Hargitay, J. Am. Chem. Soc, 76, 4492 (1954).

    Article  Google Scholar 

  30. B. G. Overell and V. Petrow, J. Chem. Soc, 1955, 232.

    Google Scholar 

  31. B. F. Erlanger and E. Brand, J. Am. Chem. Soc, 73, 3508 (1951).

    Article  Google Scholar 

  32. M. Sela, E. Katchalsky, and M. Gehatia, J. Am. Chem. Soc, 78, 746 (1956).

    Article  Google Scholar 

  33. M. Szekerke and R. Wade, Acta Chim. (Budapest), 54, 65 (1969).

    Google Scholar 

  34. M. Havranek, S. Strokrova and J. Sponar, K. Blaha, Coll. Czech. Chem Commun., 41, 3815 (1976).

    Article  Google Scholar 

  35. D. Gillessen, A. M. Felix, W. Lergier, and R. O. Studer, Helv. Chim. Acta, 53, 63 (1970).

    Article  Google Scholar 

  36. M. Sela, S. Fuchs, and R. Arnon, Biochem. J., 85, 223 (1962).

    Google Scholar 

  37. B. S. Hartley, Biochem. J., 119, 805 (1970).

    Google Scholar 

  38. F. Hudecz and G. Szokan, in: “Chromatography, the State of the Art,” H. Kalasz and L. S. Ettre, Eds., Akademia Kiado, Budapest, 1985, p. 273.

    Google Scholar 

  39. M. Pesez and J. Bartos, in: “Colorimetric and Fluorimetric Analysis of Organic Compounds and Drugs”, Dekker, New York, 1974, p. 383.

    Google Scholar 

  40. F. Hudecz, P. Kovacs, S. Kutassi-Kovacs and J. Kajtar, Colloid and Polymer Sci., 262, 208 (1984).

    Article  Google Scholar 

  41. J. C. Applequist, Ph.D. Thesis, Harvard University Press.

    Google Scholar 

  42. N. K. Jerne and A. A. Nordin, Science, 140, 405 (1963).

    Article  ADS  Google Scholar 

  43. U. H. Behling and A. Nowotny, Proc. Soc. Exp. Biol. Med., 157, 348 (1978)

    Article  Google Scholar 

  44. O. B. Zaalberg, Nature, 202, 1231 (1964).

    Article  ADS  Google Scholar 

  45. D. Gaal, F. Hudecz and M. Szekerke, J. Biol. Resp. Modifiers, 3, 174 (1984).

    Google Scholar 

  46. N. Greenfield and G. D. Fasman, Biochemistry, 8, 4108 (1969).

    Article  Google Scholar 

  47. A. Y. Adler, N. J. Greenfield and G. D. Fasman, in: “Methods in Enzymology”, Vol. XXVII, Enzyme Structure, C. H. W. Hirs, W. N. Timashoff, Eds., 1973, p 675.

    Google Scholar 

  48. V. P. Saxena and D. B. Wetlaufer, Proc. Natl. Acad. Sci. (USA), 68, 969 (1971).

    Article  ADS  Google Scholar 

  49. H. J. Goren, C. R. McMillin and A. G. Walton, Biopolymers, 16, 1527 (1977).

    Article  Google Scholar 

  50. H. J. Goren, M. Fridkin, E. Katchalski-Katzir and N. Lotan, Biopolymers, 18, 981 (1979).

    Article  Google Scholar 

  51. J. Symoens, M. Rosenthal, M. De Brabander and G. Goldstein, Springer Semin. Immunopathol., 2, 49 (1979).

    Article  Google Scholar 

  52. F. Hudecz, E. Rajnavolgyi, D. Gaal, M. Szekerke and J. Gergely, 9th. International Convocation on Immunol., Buffalo, Abstract p. 10, (1984).

    Google Scholar 

  53. E. Heber-Katz, (personal communication).

    Google Scholar 

  54. D. Gaal, F. Hudecz and M. Szekerke, 16th. Meeting of FEBS, Moscow, Abstract p. 358, (1984).

    Google Scholar 

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Author information

Authors and Affiliations

  1. The Wistar Institute of Anatomy and Biology, 36th Street at Spruce, Philadelphia, Pennsylvania, 19104, USA

    F. Hudecz

  2. Research Group for Peptide Chemistry, Hungarian Academy of Science, Eotvos L. University Institute of Organic Chemistry, H-1088 Budapest, Muzeum Krt 4/B, Budapest, Hungary

    F. Hudecz, J. Kajtar & M. Szekerke

  3. Institute of Organic Chemistry and Biochemistry, Prague, Czechoslovakia

    H. Votavova, J. Sponar & K. Blaha

  4. Research Institute of Oncopathology, National Oncological Institute, Czechoslovak Academy of Sciences, Budapest, Hungary

    D. Gaal

Authors
  1. F. Hudecz
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  2. H. Votavova
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  3. D. Gaal
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  4. J. Sponar
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  5. J. Kajtar
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  6. K. Blaha
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  7. M. Szekerke
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Editor information

Editors and Affiliations

  1. Youngstown State University, Youngstown, Ohio, USA

    Charles G. Gebelein

  2. Florida Atlantic University, Boca Raton, Florida, USA

    Charles E. Carraher Jr.

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Hudecz, F. et al. (1985). Branched Polypeptides with a Poly(L-Lysine) Backbone: Synthesis, Conformation, and Immunomodulation. In: Gebelein, C.G., Carraher, C.E. (eds) Polymeric Materials in Medication. Polymer Science and Technology, vol 32. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2245-8_22

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  • DOI: https://doi.org/10.1007/978-1-4899-2245-8_22

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-2247-2

  • Online ISBN: 978-1-4899-2245-8

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