A Note on: A Preliminary Investigation of the Influence of the Stationary Phase in Ion-Pair Thin-Layer Chromatography

  • R. J. Ruane
  • I. D. Wilson


Ion-pair (IP) reagents are routinely used for the chromatography of polar ionisable compounds in high performance liquid chromatography (HPLC). However, the application of these compounds to the separation of polar substances by thin-layer chromatography (TLC) has received much less attention (see refs 1 to 5 for some applications). In previous studies[4–8] we examined the use of quaternary ammonium IP-reagents as an aid to the TLC of some polar organic acids on a limited range of stationary phases (silica gel, C18 bonded silica gel and paraffin coated silica gel). Here we describe the results of preliminary studies undertaken using some of the other commercially available stationary phases with a range of ion-pair reagents. The phases investigated were C2, C8, aminopropyl (NH2), cyan-opropyl (CN) and diphenyl bonded silica gels, all of which were compared to a C18 bonded material for the chromatography of four aromatic acids as described below.


High Performance Liquid Chromatography Test Compound Test Acid Solvent Front Polar Substance 
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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • R. J. Ruane
    • 1
  • I. D. Wilson
    • 1
  1. 1.Department of Safety of MedicinesICI Pharmaceuticals DivisionMacclesfield CheshireUK

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