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Selective Antibody Responsive Membrane Electrodes

  • M. Y. Keating

Abstract

Demands of sensitivity, speed and accuracy of immunoassay methods in the health care industry have stimulated the interest of scientists in many disciplines. Although there exist well-established assays, alternative methods are still being sought. A considerable advance has been made in Potentiometric immuno-sensors alone. The method described in this chapter is different from straight forward enzyme labeled immuno techniques (e. g., Meyerhoff and Rechnitz, 1979; Boitieux, Desmet and Thomas, 1979; Alexander and Maitra, 1982) where an electrometrically measurable product is liberated by the enzyme label. This class of membrane electrodes respond to specific antibodies through modulation of a background potential fixed by a marker ion. Solsky and Rechnitz (1981) showed that a PVC (polyvinyl chloride) matrix containing DNP-ionophore conjugate responded to anti-DNP antibodies specifically. Keating and Rechnitz (1983, 1984) found that cortisol-ionophore and digoxin-ionophore membrane electrodes are specific to their corresponding antibodies. The term “ionophore” refers to a class of macro-organic molecules which are capable to solubilize specifically an inorganic ion of the suitable size in organic mediums. The ionophore used were benzo-crown ethers and the marker ion was potassium ion.

Keywords

Crown Ether Membrane Electrode Potentiometric Response Rabbit Serum Albumin Conjugate Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • M. Y. Keating
    • 1
  1. 1.Department of ChemistryUniversity of DelawareNewarkUSA

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