Selective Antibody Responsive Membrane Electrodes

  • M. Y. Keating


Demands of sensitivity, speed and accuracy of immunoassay methods in the health care industry have stimulated the interest of scientists in many disciplines. Although there exist well-established assays, alternative methods are still being sought. A considerable advance has been made in Potentiometric immuno-sensors alone. The method described in this chapter is different from straight forward enzyme labeled immuno techniques (e. g., Meyerhoff and Rechnitz, 1979; Boitieux, Desmet and Thomas, 1979; Alexander and Maitra, 1982) where an electrometrically measurable product is liberated by the enzyme label. This class of membrane electrodes respond to specific antibodies through modulation of a background potential fixed by a marker ion. Solsky and Rechnitz (1981) showed that a PVC (polyvinyl chloride) matrix containing DNP-ionophore conjugate responded to anti-DNP antibodies specifically. Keating and Rechnitz (1983, 1984) found that cortisol-ionophore and digoxin-ionophore membrane electrodes are specific to their corresponding antibodies. The term “ionophore” refers to a class of macro-organic molecules which are capable to solubilize specifically an inorganic ion of the suitable size in organic mediums. The ionophore used were benzo-crown ethers and the marker ion was potassium ion.


Crown Ether Membrane Electrode Potentiometric Response Rabbit Serum Albumin Conjugate Molecule 
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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • M. Y. Keating
    • 1
  1. 1.Department of ChemistryUniversity of DelawareNewarkUSA

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