Biological Activity of Non-GnRH Synthetic Peptide Sequences of the GnRH Precursor
The demonstration of GnRH biosynthesis by tryptic and carboxypeptidase B cleavage of a higher molecular precursor in sheep, pig and rat hypothalamus, and the postulate that pGlu1 is derived from cyclizafion of Gln while the amide of Gly10 is generated from an additional Gly followed by a pair of basic amino acid residues (Millar et al., 1977, 1978, 1981), was recently definitively demonstrated by nucleotide sequencing of human placental cDNA (Seeburg and Adelman, 1984) and human and rat hypothalamic cDNA (Adelman et al., 1986).
KeywordsHPLC Filtration Urea Lactate Amide
Unable to display preview. Download preview PDF.
- Millar, R. P., Denniss, P., Tobler, C., King, J. C., Schally, A. V., and Arimura, A., 1978, Presumptive prohormonal forms of hypothalamic peptide hormones, in: “Cell Biology of Hypothalamic Neurosecretion,” J. D. Vincent, and C. Kordon, eds., p. 487, Centre National de la Recherche Scientifique 280, Bordeaux.Google Scholar
- Millar, R. P., and King, J. A., 1984, Molecular heterogeneity of luteinizing hormone-releasing hormone, in: “Hormonal Control of the Hypothalamo-Pituitary-Gonadal Axis,” K. W. McKerns, ed., p. 437, Plenum Publishing Corporation, New York.Google Scholar
- Millar, R. P., Wegener, I., and Schally, A. V., 1981, Putative prohormone of luteinizing hormone-releasing hormone, in: “Neuropeptides: Biochemical and Physiological Studies,” R, P. Millar, ed., p. 111, Churchill-Livingstone, New York.Google Scholar
- Schally, A. V., Olsen, D. B., Gulyas, J., Szoke, B., Horvath, J., Karashima, T., Redding, T. W., Nikolics, K., and Seeburg, P. H., 1986, In vitro and in vivo studies with synthetic gonadotropin releasing hormone-associated peptide (GAP), a proposed prolactin release-inhibiting peptide, Proc. 68th Ann. Meeting Endocr. Soc, Anaheim, p. 26.Google Scholar
- Wormald, P., Milton, R. deL., Abrahamson, M., Duflou, J., Roberts, J., Brandt, W., and Millar, R., 1985, A peptide fragment from the GnRH precursor stimulates gonadotropin release from cultured human pituitary cells, S. Afr. J. Sci., 81:579.Google Scholar