Thermal Recovery of Cells Cultured on Poly(N-Isopropylacrylamide) Surface-Grafted Polystyrene Dishes
Surface coating by thermally reversible polymers (TRP) has been recently suggested as a new system for the recovery of cells from tissue culture substrata without the need for proteolytic enzymes (e.g. trypsin) to digest the matrix responsible for attachment1–4. In these systems, cells can be recovered from tissue culture substrata simply by lowering the temperature below a critical threshold. TRPs suggested for use in cell culture technology exhibit, in the presence of water, a lower critical solution temperature (LCST). Above the LCST, they have a low water content and a compact chain structure, but below the LCST they swell greatly and exhibit an extended chain conformation. While the former surface structure can support cell attachment and growth, the high hydrophilicity and water content of the latter prevent cell attachment5.
KeywordsMercury EDTA Hydrocarbon Penicillin Oligomer
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