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Thermal Recovery of Cells Cultured on Poly(N-Isopropylacrylamide) Surface-Grafted Polystyrene Dishes

  • Marco Morra
  • Clara Cassinelli

Abstract

Surface coating by thermally reversible polymers (TRP) has been recently suggested as a new system for the recovery of cells from tissue culture substrata without the need for proteolytic enzymes (e.g. trypsin) to digest the matrix responsible for attachment1–4. In these systems, cells can be recovered from tissue culture substrata simply by lowering the temperature below a critical threshold. TRPs suggested for use in cell culture technology exhibit, in the presence of water, a lower critical solution temperature (LCST). Above the LCST, they have a low water content and a compact chain structure, but below the LCST they swell greatly and exhibit an extended chain conformation. While the former surface structure can support cell attachment and growth, the high hydrophilicity and water content of the latter prevent cell attachment5.

Keywords

Lower Critical Solution Temperature Cell Recovery Thermal Recovery Surface Modification Technique Support Cell Attachment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Marco Morra
    • 1
  • Clara Cassinelli
    • 1
  1. 1.Nobil Bio RicercheVillafranca d’AstiItaly

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