Abstract
The first data to demonstrate glucose transporter translocation in muscle used membranes enriched in sarcolemma because it was assumed that this was the equivalent of the cell membrane of adipocytes. We studied translocation in intact human muscle using immunogold labeling of the GLUT4 transporter but found very little labeling on the sarcolemma. In contrast, there was abundant gold-labeling associated with the T-tubules and we proposed that glucose transport occured across this membrane system. In a subsequent study using an entirely different technique, we labeled cell surface glucose transporters of rat muscle with a cell impermeant photolabel and demonstrated that a majority of the glucose transporters were translocated to T-tubules, not to the sarcolemma, in response to insulin. In this report we show for the first time that in insulin-plus contraction stimulated muscle, GLUT4 glucose transporters are associated with an area that we call the SCT complex (Sarcolemmal, Caveoli, T-tubule complex). This SCT complex may play an important role in delivering metabolites to the muscle under conditions, such as muscle contraction, when there is a very high requirement for glucose transport.
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References
Burdett, E., T. Beeler, and A. Klip. Distribution of glucose transporters and insulin receptors in the plasma membrane and transverse tubules of skeletal muscle. Arch. Biochem. Biophys. 253: 279–286, 1987.
Cullen, J.J., S. Hollingworth, and M.W. Marshall. A comparative study of the transverse tubular system of the rat extensor digitorum longus and soleus muscles. J. Anat. 138: 297–308, 1984.
Dohm, G.L., P.L. Dolan, W.R. Frisell and R.W. Dudek. Role of transverse tubules in insulin stimulated muscle glucose transport. J. Cell. Biochem. 52: 1–7, 1993.
Dohm, G.L., G.J. Kasperek, E.B. Tapscott and G.R. Beecher. Effect of exercise on synthesis and degradation of muscle protein. Biochem. J. 188: 255–262, 1980.
Dombrowski, L. and A. Marette. Marked depletion of GLUT4 glucose transporters in transverse tubules of skeletal muscle from streptozotocin-induced diabetic rats. FEBS Lett 374: 43–47, 1995.
Dombrowski, L., D. Roy, B. Morcotte, and A. Marette. A new procedure for the isolation of plasma membranes, T-tubules, and internal membranes from skeletal muscle. Am. J. Physiol. 270: E667–E676, 1996.
Dudek, R.W., G.L. Dohm, G.D. Holman, S.W. Cushmanand, CM. Wilson. Glucose transporter localization in rat skeletal muscle: autoradiographic study using ATB-[2-3H] BMPA photolabel. FEBS Lett 339: 205–208, 1994.
Friedman, J.E., R.W. Dudek, D.S. Whitehead, D.L. Downes, W.R. Frisell, J.F. Caro and G.L. Dohm. Immunolocalization of glucose transporer GLUT-4 within human skeletal muscle. Diabetes 40: 150–154, 1991.
Fushiki, T., J.A. Wells, E.B. Tapscott and G.L. Dohm. Changes in glucose transporters in muscle in response to exercise. Am. J. Physiol. 256: E580–E587, 1989.
Houmard, J.A., M.S. Hickey, G.L. Tyndall, K.E. Gavigan and G.L. Dohm. Seven days of exercise increases GLUT4 protein content in human skeletal muscle. J. Appl. Physiol 79: 1936–1938, 1995.
Klip, A., T. Randal, D.A. Young, and J.O. Holloszy. Insulin-induced translocation of glucose transporters in rat hindlimb muscles. FEBS Lett. 224: 224–230, 1987.
Marette, A., E. Burdett, A. Douen, M. Vranic and A. Klip. Insulin induces the translocation of GLUT4 from a unique intracellular organelle to transverse tubules in rat skeletal muscle. Diabetes 41: 1562–1569, 1992.
Munoz, P., M. Rosemblatt, X. Testar, M. Palacin, and A. Zorzano. Isolation and characterization of distinct domains of sarcolemma and T-tubules from rat skeletal muscle. Biochem. J. 307: 273–280, 1995.
Munoz, P., M. Rosemblatt, X Testar, M. Palacin, G. Thoidis, P.F. Pilch and A. Zorzano. The T-tubule is a cell-surface target for insulin-regulated recycling of membrane proteins in skeletal muscle. Biochem. J. 312: 393–400, 1995.
Roy, D. and A. Marette. Exercise induces the translocation of GLUT4 to transverse tubules from an intracellular pool in rat skeletal muscle. Biochem. Biophys. Res. Commun. 223: 147–152, 1996.
Saborido, A., F. Molano, G. Moro, A. Megias. Regulation of dihydropyridine receptor levels in skeletal and cardiac muscle by exercise training. Pflugers Arch-Eur. J. Physiol. 429: 364–369, 1995.
Wang, W., P.A. Hansen, B.A. Marshall, J.O. Holloszy, and M. Mueckler. Insulin unmasks a COOH-terminal GLUT4 epitope and increases glucose transport across T-tubules in skeletal muscle. J. Cell Biol. 135: 415–430, 1996.
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Dohm, G.L., Dudek, R.W. (1998). Role of Transverse Tubules (T-Tubules) in Muscle Glucose Transport. In: Richter, E.A., Kiens, B., Galbo, H., Saltin, B. (eds) Skeletal Muscle Metabolism in Exercise and Diabetes. Advances in Experimental Medicine and Biology, vol 441. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1928-1_3
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DOI: https://doi.org/10.1007/978-1-4899-1928-1_3
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