Abstract
The introduction of solid-phase methods has had great impact on molecular biology in the area of synthesis and sequencing of proteins and DNA. High yield and reproducibility can be obtained, and automation is facilitated since reaction buffers can be rapidly and easily changed. There are many alternative solid supports, such as organic or inorganic polymer beads or the walls of test tubes or microtiter wells. An attractive alternative is the use of monosized magnetic particles, Dynabeads (Lea et al., 1988; Ugelstad et al., this volume, Chapter 16), which combines the convenience of magnetic motility with an adsorption and desorption of biomolecules at the surface. This provides reaction kinetics similar to those found in a free solution. Magnetic beads with covalently bound streptavidin can be used for directed immobilization of both double-stranded and single-stranded biotinylated DNA. The remarkable stability and strength of the biotin-streptavidin interaction (K d = 10−15 M) allow DNA manipulations, such as strand melting, elution, and hybridization (using alkali, temperature, or formamide) to be performed without interfering with the binding of the DNA to the beads.
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© 1993 Springer Science+Business Media New York
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Uhlén, M., Olsvik, Ø., Hornes, E. (1993). Affinity Separation of Nucleic Acids on Monosized Magnetic Beads. In: Ngo, T.T. (eds) Molecular Interactions in Bioseparations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1872-7_31
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DOI: https://doi.org/10.1007/978-1-4899-1872-7_31
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