Affinity Chromatography Using Immobilized Antisense-Family Peptides
Antisense peptides are sequences of amino acids encoded in the antisense strand of DNA. These peptides normally are not expressed in cells. Nonetheless, as proposed originally by Mekler (1969), such peptides can bind to the corresponding sense peptides, the sequences encoded in the DNA complementary to the antisense strand (Fig. 1A). This was first shown experimentally by Bost et al. (1985) with adrenocorticotropin and the corresponding chemically synthesized antisense peptide. Subsequently, many other experimental observations of antisense peptide recognition of sense peptides have been reported [for reviews, see Brentani (1988), Blalock (1990), Chaiken (1992), and Tropsha et al. (1992); see also several recently published papers (Bajpai et al., 1991; Gartner et al., 1991; Fassina and Cassani, 1992; Fassina et al., 1992a,b,c)].
KeywordsAntisense Strand Affinity Ligand Affinity Matrix Peptide Recognition Complementary Peptide
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