Isolated Perfused Porcine Skin Flap Systems
The ultimate goal of an in vitro model system is to predict the behavior of the drug in the in vivo setting. Most in vitro skin models used to assess the percutaneous absorption or transdermal delivery of topically applied drugs are designed around the assumption that the stratum corneum and/or epidermis is the principal barrier to compound penetration through skin. Thus, all such models were usually avascular and, until recently, nonviable preparations. However, in vivo, this stratum corneum and living epidermal barrier resides on a well-vascularized and metabolically active dermal substrate offering ample biological targets for specific types of drugs. Drugs may be vasoactive and modulate the cutaneous microcirculation; they may serve as substrates of epidermal and dermal drug-metabolizing enzymes, or they may activate cytokine and/or other modulator receptors which result in changes in epidermal, dermal, or endothelial cell function. Therefore, the anatomical and physiological complexity of human skin is significantly greater than that modeled in existing in vitro systems, which thus do not afford the possibility of detecting these potential biologically relevant events.
KeywordsStratum Corneum Pharmacokinetic Model Skin Flap Luteinizing Hormone Release Hormone Percutaneous Absorption
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