Summary
We have identified, purified to apparent homogeneity and chemically characterized a biologically-relevant porcine enterocyte receptor for group A porcine rotavirus. Ceramide glycanase digestion followed by acid hydrolysis and monosaccharide compositional analyses indicated the receptor is a family of two GM3 gangliosides, one containing N-glycolyl-neuraminic acid and the other N-acetylneuraminic acid. Both gangliosides displayed dose-dependent inhibition of rotavirus binding to, and infectivity of, host cells. Inhibition of infectivity in a focus-forming-unit-reduction assay was achieved with as little as 2 nmols of NeuGcGM3 (50% inhibition with 3.97 nmol) or NeuAcGM3 (50% inhibition with 9.84 nmol) per 104 FFU of virus. Preliminary data suggest specific porcine GM3 carbohydrate fine structure or spatial orientation of the sialyloligosaccharide epitopes of the holoGM3 gangliosides may be crucial to enterocyte receptor recognition by rotavirus. We have quantified both NeuGcGM3 and NeuAcGM3 in enterocytes of various-aged pigs from newborn through 16 weeks and have found with increasing age the amount of both GM3 derivatives, especially NeuGcGM3 per gram (dry weight) intestinal brush border decreases rapidly from newborn through 4 weeks of age. These results may help explain the age-sensitivity of piglets to severe rotavirus diarrhea.
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References
Bern, C., J. Martines, 1. de Zoysa and R. I. Glass. (1992). “The magnitude of the global problem of diarrhoeal disease: a ten year update”. Bull. WHO 70: 705–714.
Blacklow, N. R. and H. B. Greenberg. (1991). “Viral gastroenteritis”. The New England Journal of Medicine 325: 252–264.
Bohl, E. H. (1979). “Rotavirus diarrhea in pigs: Brief review”. J. Am. Vet. Med. Assoc. 174: 613–615.
Centers for Disease Control and Prevention. (1992). “The management of acute diarrhea in children: Oral rehydration, maintenance, and nutritional therapy”. MMWR 41: 1–20.
Cohen, M. B. (1991). “Etiology and mechanisms of acute infectious diarrhea in the United States”. J. Pediatr. 118: S34–39.
Hayase, T., K. G. Rice, K. M. Dziegielewaska, M. Kuhlenschmidt, T. Reilly and Y. C. Lee. (1992). “Comparison of N-glycosides of fetuins from different species and human aZ HS-glycoprotein”. Biochemistry 31: 4915–4921.
Kapikian, A. Z. and R. M. Chanock. (1990). “Rotaviruses”, p. 1353–1403. In Fields, B. N. and D. M. Knipe (ed.), Virology. Raven Press, New York.
Leece, T. G., M. W. King and W. E. Dorsey. (1978). “Rearing regimen producing piglet diarrhea (rotavirus) and its relevance to acute infantile diarrhea”. Science 199: 776–778.
Powell, L. D. and G. W. Hart. (1986). “Quantitation of picomole levels of N-acetyl-and N-glycolylneuraminic acids by a HPLC-adaptation of the thiobarbituric acid assay”. Anal. Biochem. 157: 179–185.
Rolsma, M. D., H. B. Gelberg and M. S. Kuhlenschmidt. (1994). “A novel assay for evaluation of rotavirus-cell interactions: Identification of an enterocyte ganglioside receptor for procine group A rotavirus”. J. Virol. 68: 258–268.
Rotbart, H. A., W. L. Nelson, M. P. Glode, T. C. Triffon, S. J. H. Kogut, R. H. Yolken, J. A. Hernandez and M. J. Levin. (1988). “Neonatal rotavirus-associated necrotizing enterocolitis: Case control study and prospective surveillance during an outbreak”. J. Pediatr. 112: 87–93.
Saulsbury, F. T., J. A. Winkelstein and R. H. Yolken. (1980). “Chronic rotavirus infection in immunodeficiency”. J. Pediatr. 97: 61–65.
Schnaar, R. L., B. K. Brandley, L. K. Needham, P. Swank-Hill and C. C. Blackburn. (1989). “Adhesion of eukaryotic cells to immobilized carbohydrates”. Meth. Enzmol. 179: 542–558.
Vesikari, T., E. Isolauri, A. Delem, E. D’Hondt, F. E. Andre and G. Zissis. (1975). “Immunogenicity and safety of live oral attenuated bovine rotavirus vaccine strain RIT 4237 in adults and young children”. Lancet 2: 807–811.
Yolken, R. H., C. A. Bishop, R. R. Rowsend, E. A. Bolyard, J. G. Bartlett, G. W. Santos and R. Sarai. (1982). “In- fectious gastroenteritis in bone-marrow-transplant recipients”. New. Engl. J. Med. 306: 1009–1012.
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Kuhlenschmidt, M.S., Rolsma, M.D., Kuhlenschmidt, T.B., Gelberg, H.B. (1997). Characterization of a Porcine Enterocyte Receptor for Group a Rotavirus. In: Paul, P.S., Francis, D.H., Benfield, D.A. (eds) Mechanisms in the Pathogenesis of Enteric Diseases. Advances in Experimental Medicine and Biology, vol 412. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1828-4_21
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