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Development of a Group B Streptococcus (GBS) Cloning System

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Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 418)

Abstract

In order to identify genes coding for virulence factors of GBS, a reliable method of incorporating genomic DNA into a cloning system must be developed. Most molecular technologies depend on E. coli based vector-host systems, and we describe here that these methods are unsuitable for use in cloning GBS DNA.

Keywords

Wheat Germ Agglutinin Cloning System Strain MG1363 Royal Postgraduate Medical School Pronase Digestion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  1. 1.Infectious Diseases and BacteriologyRoyal Postgraduate Medical SchoolLondonUK
  2. 2.Department of ImmunologyWilhelmina KinderziekenhuisUtrechtThe Netherlands

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