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Phagocytic, Serological, and Protective Properties of Streptococcal Group A Carbohydrate Antibodies

  • J. B. Zabriskie
  • T. Poon-King
  • M. S. Blake
  • F. Michon
  • M. Yoshinaga
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 418)

Abstract

To determine whether antibodies other than the type specific protein were phagocytic and protective, we embarked on a study of group A carbohydrate (GRA-CHO) antibodies in human sera. Using a modified in vitro phagocytic assay, we determined that carbohydrate antibodies were phagocytic for several different type specific GRA streptococcal strains. The opsonic activity could be abolished by absorption with sepharose coupled N-acetyl glucosamine beads (1). Animals immunized with GRA coupled to tetanus toxoid (TT), resulted in sera with high titers to GRA-CHO. These rabbit sera were also phagocytic for multiple type specific GRA strains. Positive passive protection tests against two different M type strains were seen with these rabbit anti-sera in an in vivo mouse model challenge assay.

Keywords

Protective Property Tetanus Toxoid Rabbit Seron Opsonic Activity ELISA Titer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Salvadori L, et al. 1995. JID 171:593–600.PubMedCrossRefGoogle Scholar
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    Lancefield RC. JEM 1959. 10:271–292Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • J. B. Zabriskie
    • 1
  • T. Poon-King
    • 2
  • M. S. Blake
    • 3
  • F. Michon
    • 3
  • M. Yoshinaga
    • 4
  1. 1.Rockefeller UniversityNew YorkUSA
  2. 2.San FernandoTrinidad
  3. 3.North American VaccineBeltsvilleUSA
  4. 4.Kagoshima UniversityJapan

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