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Aggregation and Binding Substances Enhance Pathogenicity in a Rabbit Model of Enterococcus faecalis Endocarditis

  • P. M. Schlievert
  • G. M. Dunny
  • J. A. Stoehr
  • A. P. Assimacopoulos
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 418)

Abstract

We investigated the importance of Aggregation Substance (AS) and Binding Substance (BS) in a rabbit model of Enterococcus faecalis endocarditis. Aggregation substance (AS), a pheromone inducible, surface protein of E. faecalis, promotes mating aggregate formation during bacterial conjugation and is thought to be a virulence factor in enterococcal infections by promoting binding to a variety of eukaryotic cell surfaces2. AS is not produced constitutively, but may be induced by eukaryotic factors in serum. AS contains the amino acid motifs Arg-Gly-Asp-Ser and Arg-Gly-Asp-Val, found in fibronectin and other proteins which mediate adhesion to eukaryotic cell surfaces2. Binding Substance (BS) is the site recognized by AS and is also necessary for mating aggregate formation. Lipotechoic acid (LTA) inhibits aggregate formation and may function as enterococcal BS2. Because of the increasing frequency with which enterococci are identified as nosocomial pathogens and because of the increasing incidence of vancomycin and other antibiotic resistance among these organisms, greater attention is focusing on the pathogenesis of enterococcal infections. AS and BS are thought to play an important role in enterococcal infections.

Keywords

Binding Substance Enterococcus Faecalis Zealand White Rabbit Toxic Shock Syndrome Amino Acid Motif 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Chow JW, Thal LA, Perri MB, Vazquez JA, Donabedian SM, Clewell DB, Zervos MJ. Plasmid-associated hemolysin and aggregation substance production contribute to virulence in eperimental enterococcal endocarditis. Antimicrob Agent Chemother 1993;37:2474–2477.CrossRefGoogle Scholar
  2. 2.
    Olmsted SB, Kao SM, van Putte LJ, Gallo JC, Dunny GM. Role of pheromone-inducible surface protein Asc10 in mating aggregate formation and conjugal transfer of the Enterococcus faecalis plasmid pCF10. J Bacteriol 1991; 173:7665–7672.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • P. M. Schlievert
    • 1
  • G. M. Dunny
    • 1
  • J. A. Stoehr
    • 2
  • A. P. Assimacopoulos
    • 2
  1. 1.Department of MicrobiologyUniversity of Minnesota Medical SchoolMinneapolisUSA
  2. 2.St. Paul Infectious Disease AssociatesSt. PaulUSA

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