Genetic Mosaic Upstream of scpG in Human Group G Streptococci Contains Sequences from Group A Streptococcal Virulence Regulon
In group A streptococci (GAS) the genes for M protein (emm), M-like proteins (emmL) and C5a-peptidase (scpA) are closely linked, and form part of a virulence regulon which is controlled by an upstream gene, mga 3, 4. In some M types, this region also contains a gene which codes for a streptococcal inhibitor of complement mediated lysis, sic 1. Heterogeneity of the emm and emmL sequences combined with differences in the organisation of these genes within the regulon contributes to polymorphism at this locus. Both scpA and mga are ubiquitous8. Taking advantage of these findings, we have developed a polymerase chain reaction (PCR) based typing method for GAS.
Unable to display preview. Download preview PDF.
- 4.Cleary, P.P., D. LaPenta, D. Heath, E.J. Haanes, and C. Chen. (1991) In Genetics and Molecular Biology of Streptococci, Lactococci, and Enterococci. Dunriy GM, Cleary, PP and McKay LL (eds) Washington DC: American Society for Microbiology PP 147-151.Google Scholar
- 5.Cleary, PP., Peterson, J., Chen, C., and Nelson, C. (1991) Infectlmmun. 59: 2305–2310.Google Scholar