Genetic Mosaic Upstream of scpG in Human Group G Streptococci Contains Sequences from Group A Streptococcal Virulence Regulon

  • Kadaba S. Sriprakash
  • Jon Hartas
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 418)


In group A streptococci (GAS) the genes for M protein (emm), M-like proteins (emmL) and C5a-peptidase (scpA) are closely linked, and form part of a virulence regulon which is controlled by an upstream gene, mga 3, 4. In some M types, this region also contains a gene which codes for a streptococcal inhibitor of complement mediated lysis, sic 1. Heterogeneity of the emm and emmL sequences combined with differences in the organisation of these genes within the regulon contributes to polymorphism at this locus. Both scpA and mga are ubiquitous8. Taking advantage of these findings, we have developed a polymerase chain reaction (PCR) based typing method for GAS.


Polymerase Chain Reaction Primer Human Group Mosaic Structure Upstream Gene Complement Mediate Lysis 
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  1. 1.
    Akesson, P., Sjoholm, A.G., and Bjorck, L. (1996) J. Biol. Chem. 271: 1081–1088.PubMedCrossRefGoogle Scholar
  2. 2.
    Bessen, D., Jones, KF and Fischetti VA (1989) J.Exp.Med. 169:269–283.PubMedCrossRefGoogle Scholar
  3. 3.
    Caparon, M.G., and Scott J.R. (1987) Proc.Natl.Acad.Sci. USA 84: 8677–8681.PubMedCrossRefGoogle Scholar
  4. 4.
    Cleary, P.P., D. LaPenta, D. Heath, E.J. Haanes, and C. Chen. (1991) In Genetics and Molecular Biology of Streptococci, Lactococci, and Enterococci. Dunriy GM, Cleary, PP and McKay LL (eds) Washington DC: American Society for Microbiology PP 147-151.Google Scholar
  5. 5.
    Cleary, PP., Peterson, J., Chen, C., and Nelson, C. (1991) Infectlmmun. 59: 2305–2310.Google Scholar
  6. 6.
    Collins CM, Kimura A and Bisno A. (1992) Infect. Immun. 60:3689–3696.PubMedGoogle Scholar
  7. 7.
    Gardiner, D., Hartas, J., Currie, B., Mathews, J.D. Kemp, D.J., and Sriprakash, K.S. (1995) PCR Methods and applications, 4: 288–293.PubMedCrossRefGoogle Scholar
  8. 8.
    Podbielski, A. (1992) Med.Microbiol.Immunol. 181: 227–240181: 227-240.PubMedCrossRefGoogle Scholar
  9. 9.
    Sriprakash, K.S., and Hartas, J. 1996. Microb. Pathog. 20:275–285.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Kadaba S. Sriprakash
    • 1
  • Jon Hartas
    • 1
  1. 1.Menzies School of Health ResearchDarwinAustralia

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