Nitric Oxide Rather than Superoxide or Peroxynitrite Inhibits Insulin Secretion and Causes DNA Damage in HIT-T15 Cells

  • C. A. Delaney
  • J. M. Cunningham
  • M. H. L. Green
  • I. C. Green
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 426)

Abstract

We have previously demonstrated that treatment of rat islets of Langerhans with the nitric oxide donors 3-morpholinosydnonimine (SIN-1) and S-nitrosoglutathione (GSNO) inhibits insulin secretion and increases cyclic GMP, thus mimicking the effects of the cytokine interleukin-lβ [1] and [2]. We have recently found that nitric oxide, derived exogenously from nitric oxide donors or endogenously by IL-lβ induction of nitric oxide synthase, causes DNA damage in islets of Langerhans and in HIT-T15 cells when measured by the ‘comet’ assay [3].

Keywords

Agar Hydroxyl Cadmium Bromide Superoxide 

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • C. A. Delaney
    • 1
    • 2
  • J. M. Cunningham
    • 1
    • 2
  • M. H. L. Green
    • 1
    • 2
  • I. C. Green
    • 1
    • 2
  1. 1.Biochemistry Group, School of Biological SciencesUniversity of SussexFalmer, BrightonUK
  2. 2.MRC Cell Mutation UnitUniversity of SussexBrightonUK

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