A Novel Method for Prostaglandin Endoperoxide H Synthase Activity in Individual Intact Cells
Prostaglandins are key mediators of inflammation1. Prostaglandin endoperoxide H is synthesized by two related prostaglandin endoperoxide H synthases (PGHS-1 and PGHS-2, COX-1 and COX-2). PGHS-1 is a constitutive enzyme present in many but not all mammalian cells2. PGHS-2 is undetectable in most mammalian tissues, but expression of this isozyme can be induced by cytokines, growth factors, and tumor promoters3. This means that PGHS-2 will be closely related with inflammation. Therefore, a development of nonsteroidal anti-inflammatory drugs (NSAIDs) which inhibit PGHS-2 specifically is undergoing in the world. In the present paper, we will show a novel and convenient method for PGHS activity in intact individual cells.
KeywordsNIH3T3 Fibroblast Acetoxymethyl Ester Prostaglandin Endoperoxide Constitutive Enzyme Murine NIH3T3 Fibroblast
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