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A Novel Method for Prostaglandin Endoperoxide H Synthase Activity in Individual Intact Cells

  • Ikuo Morita
  • Melvin Schindler
  • David DeWitt
  • Sei-itsu Murota
  • William Smith
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 407)

Abstract

Prostaglandins are key mediators of inflammation1. Prostaglandin endoperoxide H is synthesized by two related prostaglandin endoperoxide H synthases (PGHS-1 and PGHS-2, COX-1 and COX-2). PGHS-1 is a constitutive enzyme present in many but not all mammalian cells2. PGHS-2 is undetectable in most mammalian tissues, but expression of this isozyme can be induced by cytokines, growth factors, and tumor promoters3. This means that PGHS-2 will be closely related with inflammation. Therefore, a development of nonsteroidal anti-inflammatory drugs (NSAIDs) which inhibit PGHS-2 specifically is undergoing in the world. In the present paper, we will show a novel and convenient method for PGHS activity in intact individual cells.

Keywords

NIH3T3 Fibroblast Acetoxymethyl Ester Prostaglandin Endoperoxide Constitutive Enzyme Murine NIH3T3 Fibroblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Masferrer, J.L., Zweifel, B.S., Manning, P.T., Hauser, S.D., Leavy, K.M., Smith, W.G., Isakson, P.C., Seibert, K., Proc. Natl. Acad. Sci., USA 1994; 91: 3228–3232.PubMedCrossRefGoogle Scholar
  2. 2.
    O’Neill, G.P., Ford-Hutchinson, A.W., FEBS Lett. 1993; 330: 156–160.PubMedGoogle Scholar
  3. 3.
    O’Sullivan, M.G., Chilton, F.H., HugginsJr., E.M., McCall, C.E., J. Biol. Chem. 1992; 267: 14547–14550.PubMedGoogle Scholar
  4. 4.
    Morita, I., Smith, W.L., DeWitt, D.L., Schindler, M., Biochemistry, 1995; 34: 7194–7199.PubMedCrossRefGoogle Scholar
  5. 5.
    Laneuville, O., Breuer, D.K., DeWitt, D.L., Hla, T., Funk, C.D., Smith, W.L., J. Pharmacol. Exp. Ther, 1944; 271: 927–934.Google Scholar
  6. 6.
    Morita, I., Schindler, M., Regier, M., Otto, J., Hori, T., DeWitt, D.L., Smith, W.L., J. Biol. Chem., 1995; 270: 10902–10908.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Ikuo Morita
    • 1
    • 2
  • Melvin Schindler
    • 1
    • 2
  • David DeWitt
    • 1
    • 2
  • Sei-itsu Murota
    • 1
    • 2
  • William Smith
    • 1
    • 2
  1. 1.Department of Physiological Chemistry, Graduate SchoolTokyo Medical and Dental UniversityYushima, Bunkyo-ku, TokyoJapan
  2. 2.Department of BochemistryMichigan State UniversityEast LansingUSA

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