Protein Phosphatases-1 and -2A Regulate Tumor Cell Migration, Invasion and Cytoskeletal Organization

  • M. Rita I. Young
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 407)

Abstract

The role of protein kinase A (PKA) and protein phosphatases (PP) -1 and -2A in regulating the metastatic phenotype of Lewis lung carcinoma (LLC) cells was evaluated. The metastatic LLC-LN7 cells were more motile and invasive than were nonmetastatic LLC-C8 cells. Compared to the nonmetastatic cells, the LLC-LN7 cells had increased PKA activity and a deficiency in PP-2A. Nonmetastatic LLC-C8 cells became migratory and invasive when PP-1 and P-2A activities were inhibited with okadaic acid. This stimulation of LLC-C8 motility was tempered by PKA inhibition. Also examined was if the okadaic acid-stimulated LLC-C8 motility was associated with a change in the cytoskeletal organization to that typical of metastatic cells. Treatment of nonmetastatic LLC-C8 cells with okadaic acid caused a redistribution of F-actin toward the periphery of the cells, and eventually to a loss of the filamentous actin network. All of these effects were reversed upon removal of okadaic acid. Our results show that PP-1/2A maintain reduced motility and increased cytoskeletal organization within nonmetastatic LLC cells.

Keywords

Migration Prostaglandin Indomethacin Plasminogen Rosen 

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • M. Rita I. Young
    • 1
    • 2
    • 3
  1. 1.Research Service (151-Z2)Hines V.A. HospitalHinesUSA
  2. 2.Department of PathologyLoyola University Stritch School of MedicineMaywoodUSA
  3. 3.Department of OtolaryngologyLoyola University Stritch School of MedicineMaywoodUSA

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