The Development of a Highly Specific, Sensitive Enzyme Immunoassay for Prostaglandin E1
Prostaglandin E1 (PGE1) is synthesized from DGLA, dihomo-γ-linolenic acid1. PGE1 has been shown to have a number of biological actions, including vasodilation2, proliferation of vascular smooth muscle cells3, platelet aggregation4 and has been shown to have insulin-like actions5, 6. It’s effects are induced by receptor mediated elevation of cAMP7. It is the major prostaglandin in semen8, 9. Interest in the measurement of PGE1 in a variety of biological matrices has been hampered by the lack of specific methods for the easy detection of this molecule. We report on the development of a rapid, highly specific enzyme immunoassay utilizing stable, color-coded reagents for PGE1.
KeywordsCross Reactivity Tissue Culture Medium Biological Matrice Human Saliva Binding Point
Unable to display preview. Download preview PDF.
- 1.J. Mai, et. al., Prostaglandins., (1980), 20, 187.Google Scholar
- 2.A.G. Olsson & L.A. Carlson, in “Advances in Prostaglandin and Thromboxane Research.”. (1976), Raven Press, NY, B. Samuelsson & R. Paoletti, (Eds.).Google Scholar
- 5.H.A. Haessler & J.D. Crawford., J. Clin. Invest.., (1967), 46, 1065–69.Google Scholar
- 6.D.F. Horrobin & M.S. Manku, International Prostaglandin Conference, (1979), pg 53.Google Scholar
- 8.L. Speroff, et. al., Am. J. Obstet. Gynec.., (1978), 107, 1111.Google Scholar
- 9.D. Rubin & M. Laposata., J. Biol. Chem., (1991), 226, 23618.Google Scholar
- 13.K. Green, et. al., Adv. Prostaglandin & Thromboxane Res., (1978), 5, 15.Google Scholar