The Development of a Highly Specific, Sensitive Enzyme Immunoassay for Prostaglandin E1
Prostaglandin E1 (PGE1) is synthesized from DGLA, dihomo-γ-linolenic acid1. PGE1 has been shown to have a number of biological actions, including vasodilation2, proliferation of vascular smooth muscle cells3, platelet aggregation4 and has been shown to have insulin-like actions5, 6. It’s effects are induced by receptor mediated elevation of cAMP7. It is the major prostaglandin in semen8, 9. Interest in the measurement of PGE1 in a variety of biological matrices has been hampered by the lack of specific methods for the easy detection of this molecule. We report on the development of a rapid, highly specific enzyme immunoassay utilizing stable, color-coded reagents for PGE1.
KeywordsAlbumin Hydroxide Lysine Dimethyl Sodium Hydroxide
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