The Development of a Highly Specific, Sensitive Enzyme Immunoassay for Prostaglandin E1

  • Rhonda Linsky
  • Jeanette Buckwalter
  • Nancy Schmidt
  • Barbara Scheuer
  • Russell Hart
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 433)


Prostaglandin E1 (PGE1) is synthesized from DGLA, dihomo-γ-linolenic acid1. PGE1 has been shown to have a number of biological actions, including vasodilation2, proliferation of vascular smooth muscle cells3, platelet aggregation4 and has been shown to have insulin-like actions5, 6. It’s effects are induced by receptor mediated elevation of cAMP7. It is the major prostaglandin in semen8, 9. Interest in the measurement of PGE1 in a variety of biological matrices has been hampered by the lack of specific methods for the easy detection of this molecule. We report on the development of a rapid, highly specific enzyme immunoassay utilizing stable, color-coded reagents for PGE1.


Cross Reactivity Tissue Culture Medium Biological Matrice Human Saliva Binding Point 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. Mai, et. al., Prostaglandins., (1980), 20, 187.Google Scholar
  2. 2.
    A.G. Olsson & L.A. Carlson, in “Advances in Prostaglandin and Thromboxane Research.”. (1976), Raven Press, NY, B. Samuelsson & R. Paoletti, (Eds.).Google Scholar
  3. 3.
    D.G. Cornwell, et. al, Lipids, (1979), 14, 194–207.PubMedCrossRefGoogle Scholar
  4. 4.
    A.J. Ally & D.F. Horrobin., Prostaglandins and Medicine., (1980), 4, 431–438.PubMedCrossRefGoogle Scholar
  5. 5.
    H.A. Haessler & J.D. Crawford., J. Clin. Invest.., (1967), 46, 1065–69.Google Scholar
  6. 6.
    D.F. Horrobin & M.S. Manku, International Prostaglandin Conference, (1979), pg 53.Google Scholar
  7. 7.
    N.P. Kurstjens, et. al., Biochem. Biophys. Res. Comm., (1990), 167, 1162.PubMedCrossRefGoogle Scholar
  8. 8.
    L. Speroff, et. al., Am. J. Obstet. Gynec.., (1978), 107, 1111.Google Scholar
  9. 9.
    D. Rubin & M. Laposata., J. Biol. Chem., (1991), 226, 23618.Google Scholar
  10. 10.
    K. Green, et. al., Anal. Biochem, (1973), 54, 434.PubMedCrossRefGoogle Scholar
  11. 11.
    J. Frolich, et. al, J. Clin. Invest., (1975), 55, 763.PubMedCrossRefGoogle Scholar
  12. 12.
    J.E. Shaw & P.W. Ramwell, Meth. Biochem. Anal., (1969), 17, 325.CrossRefGoogle Scholar
  13. 13.
    K. Green, et. al., Adv. Prostaglandin & Thromboxane Res., (1978), 5, 15.Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Rhonda Linsky
    • 1
  • Jeanette Buckwalter
    • 1
  • Nancy Schmidt
    • 1
  • Barbara Scheuer
    • 1
  • Russell Hart
    • 1
  1. 1.Assay designs, incAnn ArborUSA

Personalised recommendations