Abstract
In cells from the rabbit mTALH, arachidonic acid (AA) is metabolized primarily by the CYP pathway1, 2. Rabbit mTALH cells, when incubated with 14C-AA for 30 min, form products that segregate into two peaks, designated P1 and P2 based on their reverse phase HPLC retention times. Structural analysis by gas chromatography/mass spectrometry (GC/MS) disclosed three AA products generated by the CYP pathway in the mTALH: 20-hydroxyeicosatetraenoic acid (20-HETE), 20-eicosatetraenedioic acid (20-COOH-AA), and 19-HETE2. P2, the more polar peak, was comprised of 20-COOH-AA, an inhibitor of Na+-K+-ATPase, that was devoid of vasoactivity. P1 contained vasoactive material that was also capable of affecting Na+-K+-ATPase and yielded two products, 20-HETE and 19-HETE (Fig. 1).
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McGiff, J.C., Ferreri, N.R., Escalante, B.A., Carroll, M.A. (1997). Interactions of Renal Cytochrome P450 (CYP), Angiotensin (AII) and Tumor Necrosis Factor-Alpha (TNF): Implications for Ion Transport. In: Sinzinger, H., Samuelsson, B., Vane, J.R., Paoletti, R., Ramwell, P., Wong, P.YK. (eds) Recent Advances in Prostaglandin, Thromboxane, and Leukotriene Research. Advances in Experimental Medicine and Biology, vol 433. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1810-9_21
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