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Phytochemistry of Medicinal Plants pp 189–215Cite as

Applications of Liquid Chromatography-Mass Spectrometry to the Investigation of Medicinal Plants

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Part of the book series: Recent Advances in Phytochemistry ((RAPT,volume 29))

Abstract

Efficient detection and rapid characterization of natural products play an important role as analytical support in the work of phytochemists. The identification of a metabolite at the earliest stage of separation is a strategic element for guiding an efficient and selective isolation procedure. Chromatographic analyses are used to “pilot” the preparative isolation of natural products (optimization of the experimental conditions, checking the different fractions throughout the separation), and to control the final purity of the isolated compounds. For chemotaxonomic purposes, the botanical relationships between different species can be shown by Chromatographic comparison of their chemical composition. Comparison of chromatograms, used as fingerprints between authentic samples and unknowns, permits identification of drugs and/or the search for adulterated products. The selective detection of a given product in a complex mixture allows good quantitative measurement as well as precise chemotaxonomic comparison. Furthermore, in many applications it may be necessary not only to detect but also to identify compounds in extracts. With conventional detection methods, the identity of peaks can be confirmed only from their retention time and by comparison with authentic samples. In order to get more information on the metabolites of interest and detect them with satisfactory sensitivity and selectivity, a powerful and universal detection tool is needed.

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Author information

Authors and Affiliations

  1. Institut de Pharmacognosie et Phytochimie, Université de Lausanne, Bep, Ch-1015, Lausanne, Switzerland

    Jean-Luc Wolfender & Kurt Hostettmann

Authors
  1. Jean-Luc Wolfender
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  2. Kurt Hostettmann
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Editor information

Editors and Affiliations

  1. Université d’ Ottawa, Ottawa, Ontario, Canada

    John T. Arnason

  2. Universidad Nacional Autónoma de México, Coyoacán, México

    Rachel Mata

  3. University of South Florida, Tampa, Florida, USA

    John T. Romeo

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© 1995 Springer Science+Business Media New York

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Wolfender, JL., Hostettmann, K. (1995). Applications of Liquid Chromatography-Mass Spectrometry to the Investigation of Medicinal Plants. In: Arnason, J.T., Mata, R., Romeo, J.T. (eds) Phytochemistry of Medicinal Plants. Recent Advances in Phytochemistry, vol 29. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1778-2_9

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  • DOI: https://doi.org/10.1007/978-1-4899-1778-2_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1780-5

  • Online ISBN: 978-1-4899-1778-2

  • eBook Packages: Springer Book Archive

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