Abstract
Casein kinase II (protein kinase CKII) is a heterotetramer consisting of two α and two β subunits (1,2). The α subunit can be expressed as two different gene products which are present as α and/or α′, depending on the tissue and species of origin. The β subunit is a single gene product in higher eukaryotes. A comparison of the sequences from a number of different species shows the α and β subunits are highly conserved (1,3). The heterotetramer is stable and the structure is dissociable only under denaturing conditions. Thus, complete physical, chemical and functional analysis of the enzyme at the molecular level is dependent on obtaining subunits capable of reconstituting into active CKII. This reconstituted CKII must have the capacity to interact with and react to the surrounding environment in an appropriate manner.
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References
Tuazon, P.T. and Traugh, J.A. (1991) in Advances in Second Messenger and Phosphoprotein Research (Greengard, P. and Robison, G.A., eds.), Vol. 23, pp. 123–164, Raven Press, New York, NY.
Pinna, L.A. (1990) Biochim. Biophys. Acta 1054, 267–284.
Issinger, O.-G. (1993) Pharmac. Ther. 59, 1–30.
Hanks, S.K., Quinn, A.M. and Hunter, T.(1988) Science 241, 42–52.
Lin, W.-J., Tuazon, P.T. and Traugh, J.A. (1991) J. Biol. Chem. 266, 5664–5669.
Meggio, F., Donella-Deana, A., Brunati, A.M. and Pinna, L.A. (1982) FEBS Lett. 141, 257–262.
Hu, E. and Rubin, CS. (1990) J. Biol. Chem. 265, 20509–20615.
Grankowski, N., Boldyreff, B. and Issinger, O-G. (1991) Eur. J. Biochem. 198, 25–30.
Lin, W-J. and Traugh, J.A. (1993) Protein Express. Purific. 4, 256–264.
Jakobi, R. and Traugh, J.A. (1992) J. Biol. Chem. 267, 23894–23902.
Buchner, J. and Rudolph, R. (1991) Bio/Technology 9, 157–162.
Hathaway, G.M., Lundak, T.S., Tahara, S.M. and Traugh, J.A. (1979) Methods Enzymol. 60, 495–511.
Palen, E. and Traugh, J.A. (1991) Biochemistry, 30, 5586–5590.
Jakobi, R. and Pyerin, W. (1989) Eur. J. Biochem. 183, 227–233.
Lin, W-J., Sheu, G-T. and Traugh, J.A. (1994) Biochemistry 33, 6998–7004.
Studier, F.W., Rosenberg, A.H., Dunn, J.J. and Dubendorff, J.W. (1990) Methods Enzymol. 185, 60–89.
Tabor, S. and Brent, R. (1990) in Current Protocols in Molecular Biology (Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K., eds) pp. 16.2.1–16.2.11, Greene Publishing and Wiley-Interscience, New York, NY.
Lin, W-J., Jakobi, R. and Traugh, J.A. (1994) J. Prot. Chem. 13, 217–225.
Glover, C.V.C., Shelton, E.R. and Brutlag, D.L. (1983) J. Biol. Chem. 258, 3258–3265.
Hathaway, G.M. and Traugh, J.A. (1979) J. Biol. Chem. 254, 762–768.
Hathaway, G.M. and Traugh, J.A. (1984) Arch. Biochem. Biophys. 233, 133–138.
Jakobi, R. and Traugh, J.A. (1995) Eur. J. Biochem. 230, 111–117.
Rudolph, R. (1990) in Modern Methods in Protein and Nucleic Acid Analysis. (Tschesche, H., ed.), pp. 149–171, Walter de Gruyter, Berlin and New York, NY.
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Lin, WJ., Jakobi, R., Traugh, J.A. (1996). Renaturation and Reconstitution of Functional Holoenzyme From Recombinant Subunits of Casein Kinase II Expressed as Inclusion Bodies in E. Coli . In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering, vol 18. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1766-9_7
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DOI: https://doi.org/10.1007/978-1-4899-1766-9_7
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