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Investigational Approaches for Studying the Structures and Biological Functions of Myeloid Antimicrobial Peptides

  • Michael E. Selsted
Chapter
Part of the Genetic Engineering: Principles and Methods book series (GEPM, volume 15)

Abstract

Macrophages and neutrophils are phagocytic leukocytes of myeloid lineage, arising from hematopoietic precursors in the bone marrow. Neutrophils are short-lived cells which are terminally differentiated as they enter the circulation, whereas macrophages derive from circulating monocytes which mature into specialized cells in various tissues including lung, liver and skin. The phagocytic and microbicidal functions of neutrophils and macrophages provide an essential component of the host’s defense against colonization by a diverse spectrum of potential pathogens.

Keywords

Antimicrobial Peptide Solid Phase Peptide Synthesis Crude Peptide Virucidal Activity Arginine Side Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Thomas, E.L., Lehrer, R.I. and Rest, R.F. (1988) Rev. Infect. Dis. 10(suppl. 2), S450–S456.PubMedCrossRefGoogle Scholar
  2. 2.
    Lehrer, R.I. and Ganz, T. (1990) Blood 76, 2169–2181.PubMedGoogle Scholar
  3. 3.
    Zeya, H.I. and Spitznagel, J.K. (1963) Science 142, 1085–1087.PubMedCrossRefGoogle Scholar
  4. 4.
    Zeya, H.I. and Spitznagel, J.K. (1966) J. Bacteriol. 91, 750–754.PubMedGoogle Scholar
  5. 5.
    Zeya, H.I. and Spitznagel, J.K. (1968) J. Exp. Med. 127, 927–939.PubMedCrossRefGoogle Scholar
  6. 6.
    Ganz, T., Selsted, M.E. and Lehrer, R.I. (1990) Eur. J. Haematol. 44, 1–8.PubMedCrossRefGoogle Scholar
  7. 7.
    Gabay, J.E., Scott, R.W., Campanelli, D., Griffith, J., Wilde, C., Marra, M.N., Seeger, M. and Nathan, C.F. (1989) Proc. Nat. Acad. Sci. U.S.A. 86, 5610–5614.CrossRefGoogle Scholar
  8. 8.
    Spitznagel, J.K. (1990) J. Clin. Invest. 86, 1381–1386.PubMedCrossRefGoogle Scholar
  9. 9.
    Elsbach, P. (1990) Tib Tech 8, 26–30.CrossRefGoogle Scholar
  10. 10.
    Gennaro, R., Romeo, D., Skerlavaj, B. and Zanetti, M. (1989) in Blood Cell Biochemistry (Harris, J.R., ed.), pp. 335–368, Plenum Press, New York, NY.Google Scholar
  11. 11.
    Selsted, M.E., Tang, Y.-Q., Morris, W.L., McGuire, P.A., Novotny, M.J., Smith, W., Henschen, A.H. and Cullor, J.S. (1992) (in press).Google Scholar
  12. 12.
    Tang, Y.-Q. and Selsted, M.E. (1992). Characterization of the disulfide motif in BNBD-12, an antimicrobial beta-defensin peptide from bovine neutrophils (in press).Google Scholar
  13. 13.
    Selsted, M.E., Novotny, M.J., Morris, W.L., Smith, W. and Cullor, J.S. (1992) J. Biol. Chem. 267, 4292–4295.PubMedGoogle Scholar
  14. 14.
    Ganz, T., Rayner, J.R., Valore, E.V., Tumolo, A., Talmadge, K. and Fuller, F. (1989) J. Immunol. 143, 1358–1365.PubMedGoogle Scholar
  15. 15.
    Daher, K.A., Lehrer, R.I., Ganz, T. and Kronenberg, M. (1988) Proc. Nat. Acad. Sci. U.S.A. 85, 7327–7331.CrossRefGoogle Scholar
  16. 16.
    Sparkes, R.S., Kronenberg, M., Heinzmann, C., Daher, K.A., Klisak, I., Ganz, T. and Mohandas, T. (1989) Genomics 5, 240–244.PubMedCrossRefGoogle Scholar
  17. 17.
    Michaelson, D., Rayner, J., Couto, M. and Ganz, T. (1992) J. Leuk. Biol. 51, 634–639.Google Scholar
  18. 18.
    Borregard, N., Heiple, J.M., Simons, E.R. and Clark, R.A. (1983) J. Cell. Biol. 97, 52–61.CrossRefGoogle Scholar
  19. 19.
    Hultmark, D., Engstrom, A., Andersson, K., Steiner, H., Bennich, H. and Boman, H.G. (1983) EMBO J. 2, 571–576.PubMedGoogle Scholar
  20. 20.
    Lehrer, R.I., Rosenman, M., Harwig, S.S.L., Jackson, R. and Eisenhauer, P. (1991) J. Immunol. Meth. 137, 167–173.CrossRefGoogle Scholar
  21. 21.
    Selsted, M.E., Szklarek, D. and Lehrer, R.I. (1984) Infect. Immun. 45, 150–154.PubMedGoogle Scholar
  22. 22.
    Selsted, M.E. and Becker, H.W., III (1986) Anal. Biochem. 155, 270–274.PubMedCrossRefGoogle Scholar
  23. 23.
    Lichtenstein, A., Ganz, T., Selsted, M.E. and Lehrer, R.I. (1986) Blood 68, 1407–1410.PubMedGoogle Scholar
  24. 24.
    Selsted, M.E., Brown, D.M., DeLange, R.J. and Lehrer, R.I. (1985) J. Biol. Chem. 260, 4579–4584.PubMedGoogle Scholar
  25. 25.
    Ganz, T., Selsted, M.E., Szklarek, D., Harwig, S.S.L., Daher, K. and Lehrer, R.I. (1985) J. Clin. Invest. 76, 1427–1435.PubMedCrossRefGoogle Scholar
  26. 26.
    Selsted, M.E. and Harwig, S.S.L. (1987) Infect. Immun. 55, 2281–2286.PubMedGoogle Scholar
  27. 27.
    Eisenhauer, P.B., Harwig, S.S.L., Szklarek, D., Ganz, T., Selsted, M.E. and Lehrer, R.I. (1989) Infect. Immun. 57, 2021–2027.PubMedGoogle Scholar
  28. 28.
    Selsted, M.E., Brown, D.M., DeLange, R.J. and Lehrer, R.I. (1983) J. Biol. Chem. 258, 14485–14489.PubMedGoogle Scholar
  29. 29.
    Selsted, M.E. and Harwig, S.S.L. (1989) J. Biol. Chem. 264, 4003–4007.PubMedGoogle Scholar
  30. 30.
    Pardi, A., Hare, D.R., Selsted, M.E., Morrison, R.D., Bassolino, D.A. and Bach, A.C., (1988) J. Mol. Biol. 201, 625–636.PubMedCrossRefGoogle Scholar
  31. 31.
    Pardi, A., Selsted, M.E., Yip, P.F., Skalicky, J.J. and Zhang, X.L. (1992) Biochemistry (in press).Google Scholar
  32. 32.
    Zhang, X.L., Selsted, M.E. and Pardi, A. (1992) Biochemistry (in press).Google Scholar
  33. 33.
    Hill, C.P., Yee, J., Selsted, M.E. and Eisenberg, D. (1991) Science 251, 1481–1485.PubMedCrossRefGoogle Scholar
  34. 34.
    Ganz, T., Sherman, M.P., Selsted, M.E. and Lehrer, R.I. (1985) Amer. Rev. Respir. Dis. 132, 901–904.Google Scholar
  35. 35.
    Selsted, M.E., Miller, S.I., Henschen, A.H. and Ouellette, A.J. (1992) J. Cell. Biol. 118, 929–936.PubMedCrossRefGoogle Scholar
  36. 36.
    Kent, S.B.H. (1988) Annu. Rev. Biochem. 57, 957–989.PubMedCrossRefGoogle Scholar
  37. 37.
    Barany, G., Sole, N.A., Van Abel, R.J., Albericio, F. and Selsted, M.E. (1992) in Innovation and Perspectives in Solid Phase Synthesis and Related Technologies: Peptides, Polypeptides and Oligonucleotides (Epton, R., ed.), pp. 29–38, Intercept, Andover, England.Google Scholar
  38. 38.
    Albericio, F., Kneib-Cordonier, N., Biancalana, S., Gera, L., Masad, R.I., Hudson, D. and Barany, G. (1990) J. Org. Chem. 55, 3730–3743.CrossRefGoogle Scholar
  39. 39.
    Ouellette, A.J., Greco, R.M., James, M., Frederick, D., Naftilan, J. and Fallon, J.T. (1989) J. Cell. Biol. 108, 1687–1695.PubMedCrossRefGoogle Scholar
  40. 40.
    Selsted, M.E., Levy, J.N., Van Abel, R.J., Cullor, J.S., Bontems, R.J. and Barany, G. (1992) in Peptides: Chemistry and Biology (Smith, J.A. and Rivier, J.E., eds.), pp. 905–907, ESCOM, Leiden.Google Scholar
  41. 41.
    Selsted, M.E., Szklarek, D., Ganz, T. and Lehrer, R.I. (1985) Infect. Immun. 49, 202–206.PubMedGoogle Scholar
  42. 42.
    Lehrer, R.I., Barton, A., Daher, K.A., Harwig, S.S.L., Ganz, T. and Selsted, M.E. (1989) J. Clin. Invest. 84, 553–561.PubMedCrossRefGoogle Scholar
  43. 43.
    Daher, K., Selsted, M.E. and Lehrer, R.I. (1986) J. Virol. 60, 1068–1074.PubMedGoogle Scholar
  44. 44.
    Lehrer, R.I., Daher, K., Ganz, T. and Selsted, M.E. (1985) J. Virol. 54, 467–472.PubMedGoogle Scholar
  45. 45.
    Lichtenstein, A.K., Ganz, T., Nguyen, T., Selsted, M.E. and Lehrer, R.I. (1988) J. Immunol. 140, 2686–2694.PubMedGoogle Scholar
  46. 46.
    Lichtenstein, A. (1991) J. Clin. Invest. 88, 93–100.PubMedCrossRefGoogle Scholar
  47. 47.
    Gera, J.F. and Lichtenstein, A. (1991) Cell. Immunol. 138, 108–120.PubMedCrossRefGoogle Scholar
  48. 48.
    Kagan, B.L., Selsted, M.E., Ganz, T. and Lehrer, R.I. (1990) Proc. Nat. Acad. Sci. U.S.A. 87, 210–214.CrossRefGoogle Scholar
  49. 49.
    Territo, M.C., Ganz, T., Selsted, M.E. and Lehrer, R.I. (1989) J. Clin. Invest. 84, 2017–2020.PubMedCrossRefGoogle Scholar
  50. 50.
    Solomon, S., Hu, J., Zhu, Q., Belcourt, D., Bennett, H.P.J., Bateman, A. and Antakly, T. (1991) J. Steroid Biochem. Molec. Biol. 40, 391–398.PubMedCrossRefGoogle Scholar
  51. 51.
    Diamond, G., Zasloff, M., Eck, H., Brasseur, M., Maloy, W.L. and Bevins, C.L. (1991) Proc. Nat. Acad. Sci. U.S.A. 88, 3952–3956.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Michael E. Selsted
    • 1
  1. 1.Departments of Pathology and Microbiology and Molecular Genetics, College of MedicineUniversity of CaliforniaIrvineUSA

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