Abstract
Lithium niobate is a piezoelectric substrate of interest for applications as an electro-acoustic immunosensor. The activation of lithium niobate substrates with antibody films is carried out by using the standard immobilization technique, i.e. silanization with 3-aminopropyltriethoxysilane prior to linkage of antibody molecules via a glutaraldehyde crosslinker. The cleaning of the substrate prior to silanization was achieved by microwave and ozone/uv cleaning. X-ray photoelectron spectra show a significant reduction of impurities compared to cleaning with organic solvents only. Silanization was performed in toluene solution, toluene vapour, and by chemical vapour deposition. The differences between the various silanization processes for antigen binding examined by enzyme linked immunosorbent assay are small. Using protein G as an immobilized binding molecule for antibodies the binding capacity was enhanced by 40%.
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© 1992 Springer Science+Business Media New York
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Seeger, S., Bierbaum, K., Dahint, R., Feng, C.L., Mantar, M., Grunze, M. (1992). Preparation and Characterization of Antibody Films on Lithium Niobate Surfaces. In: Schnur, J.M., Peckerar, M., Stratton, H.M. (eds) Synthetic Microstructures in Biological Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1630-3_5
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DOI: https://doi.org/10.1007/978-1-4899-1630-3_5
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