Mapping of Gastrulation Movements in Birds

  • Lucien C. A. Vakaet
  • Hilde Bortier
Part of the NATO ASI Series book series (NSSA, volume 279)

Abstract

Mapping gastrulation movements in Birds is theoretically impossible: a map is a still image and shows at best a snapshot of gastrulation. A map cannot show movements even when it is armed with arrows: to that end movies are needed. To enable the registration and reproduction of the movements within avian blastoderms during the first 24 hours of incubation, a cinephotomicrographic device was set up. The results of these observations are preceded by a demonstration of the procedure for preparing the germs. This procedure is derived from that of New (1955). Its main characteristic is that it allows normal development in vitro for a 24 hour period in laid blastoderms that are cultivated with their ventral side up, towards the observer.

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References

  1. Callebaut, M., Van Nueten, E., 1994, Rauber’s (Koller’s) Sickle: The Early Gastrulation Organizer of the Avian Blastoderm, European Journal of Morphology, 32, 35–48.PubMedGoogle Scholar
  2. Gallera, J., 1965, Excision et transplantation des diffé rentes ré gions de la ligne primitive chez le poulet, C. R. Ass. Anat, 125, 632–639.Google Scholar
  3. Gräper, L., 1929, Die Primitiventwicklung des Hü hnchens nach stereomikrographischen Untersuchungen, kontrolliert durch vitale Farbmarkierung und verglichen mit der Entwicklung anderer Wirbeltiere, Roux’ Archiv, 116, 382–429.CrossRefGoogle Scholar
  4. Koller, C., 1882, Untersuchungen ü ber die Blä tterbildung im Hü hnerkeim, Archiv fü r Mikroskopische Anatomie, 20, 174–211.Google Scholar
  5. Merbach, H., 1935, Beobachtungen an der Keimscheibe des Hü hnchens vor dem Erscheinen des Primitivstreifens, Zeitschrift fü r Anatomie und Entwicklungsgeschichte, 104, 635–652.CrossRefGoogle Scholar
  6. New, DAT., 1955, A new technique for the cultivation of the chick embryo in vitro. J.Embrol. Exp. Morph., 7, 146–164.Google Scholar
  7. Rauber, A., 1877, Über die Stellung des Hü hnchens im Entwicklungsplan, Leipzig.Google Scholar
  8. Schoenwolf, G., Bortier, H., Vakaet, L., 1989, Fate mapping the avian Neural Plate with Quail-Chick Chimeras: Origin of Prospective Median Wedge Cells, J. Exp. Zool., 249, 271–278.PubMedCrossRefGoogle Scholar
  9. Settle, G.W., 1954, Localization of the erythrocyte-forming areas in the early chick blastoderm cultivated in vitro, Contrib. Embryol. n° 241 Carn.Inst. 50, 223–227.Google Scholar
  10. Vakaet L., 1970, Cinephotomicrographic investigations of gastrulation in the chick blastoderm, Arch. Biol (Liè ge), 81/3, 387–426.Google Scholar
  11. Waddington, C.H., 1933, Induction by the Endoderm in Birds, Philos. Trans. Roy. Soc., ser. B, 221, 179–230.CrossRefGoogle Scholar
  12. Zagris N., 1979, Differentiation capacity of unincubated chick blastoderm in culture, J. Emb. Exp. Morph. 50, 47–55.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Lucien C. A. Vakaet
    • 1
  • Hilde Bortier
    • 2
  1. 1.Laboratory of Experimental CancerologyUniversity HospitalGentBelgium
  2. 2.Department of Anatomy and NeurobiologyUniversity of Salt lake CityUtahUSA

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