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Automated C-Terminal Sequencing of Peptides and Proteins

  • Jerome M. Bailey
  • Miro Rusnak
  • John E. Shively

Abstract

The development of a chemical method for the sequential degradation of a protein or peptide from the carboxy-terminus is a goal of our laboratory. Such a method, in addition to complementing existing N-terminal methods of degradation, would be invaluable for the sequence analysis of proteins with naturally occurring N-terminal blocking groups and for the detection of post-translational processing at the carboxy-terminus of expressed gene products. Although several methods for a sequential C-terminal degradation have been proposed (Ward, 1986; Rangarajan, 1988), the thiocyanate method based on the procedure originally published by Schlack and Kumpf (1926) has been the most widely studied. Recent work in our laboratory, introducing new reagents for the derivatization of the C-terminal amino acid (Hawke et al., 1987) and for the specific cleavage of the derivatized amino acid (Bailey and Shively, 1991; Bailey et al., 1992), has suggested that an automated chemical method for the sequential degradation of polypeptides from the carboxy-terminus, analogous to the Edman method for amino terminal degradation, may be feasible.

Keywords

Acetic Anhydride Derivatized Amino Acid Amino Acid Ester Continuous Flow Reactor Ethyl Chloroformate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Jerome M. Bailey
    • 1
  • Miro Rusnak
    • 1
  • John E. Shively
    • 1
  1. 1.Division of ImmunologyBeckman Research Institute of the City of HopeDuarteUSA

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