Abstract
Automated image processing has facilitated new insight into complex data; for example it is no longer necessary to view a three-dimensional specimen as a series of two-dimensional slices. A three-dimensional image can be reconstructed, and this new image can be electronically rotated or subsections of it studied at high resolution, although a more common application is automated statistical characterization of a complex two-dimensional image. Parameters can be measured and aspect statistics calculated on a large enough population to make such numerical characterization impossible by manual means. We must recall the concept of empty magnification versus resolution when we hear that it is possible to magnify up to one thousand or more by video microscopy with a low-resolution objective. Classical principles still apply to these new techniques.(1)
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References
Tim Studt, “Add Video to Your Microscope for Even Better Imaging,” R & D Magazine (July 1992), 32–36.
Shinya Inoué, Video Microscopy ( New York: Plenum, 1986 ).
John C. Russ, The Image Processing Handbook ( Ann Arbor, MI: CRC Press, 1992 ).
a. Photometrics, Ltd., 3440 East Britannia Dr., Tucson, AZ 85708.
John C. Russ, Computer-Assisted Microscopy ( New York: Plenum, 1990 ).
R. H. Webb and C. K. Dorey, “The Pixelated Image”, in Handbook of Biological Confocal Microscopy, J. B. Pawley, ed. ( New York: Plenum, 1990 ), 41–51.
Robert Keeler, “Shop Like a Pro for Your Image Analysis System,” R & D Magazine (March 1991), 48–52.
M. Richardson, “Confocal Microscopy and 3-D Visualization,” American Laboratory (Nov. 1990), 19–24.
The Imagist 1 2 (1991).
In Focus, The Magazine for Instant Scientific Imaging 3 (Spring/Summer 1991).
D. J. Palatini, “Distributing Images to Remote Sites Using a Computer Network,” EMSA Bulletin 21, 1 (spring 1991 ).
“The Computer Corner,” EMSA Bulletin 22 3 (Nov. 1992), 78–81.
David Hessler et al.,“SYNU: Software for Visualization of 3-Dimensional Biological Structures,” Microscopy: The Key Research Tool,(Mar. 1992), 73–82.
D.-P. Hader, ed., Image Analysis in Biology, ( Boca Raton, FL: CRC Press, 1992 ).
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© 1994 Springer Science+Business Media New York
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Rochow, T.G., Tucker, P.A. (1994). Image Collection, Analysis, and Reconstruction by Computer. In: Introduction to Microscopy by Means of Light, Electrons, X Rays, or Acoustics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1513-9_19
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DOI: https://doi.org/10.1007/978-1-4899-1513-9_19
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