This chapter will review the physical principles of confocal scanning microscopy. The ability to modify and improve the optical imaging in these instruments results mainly from adoption of a scanning approach. In this way the only requirement of the optical system is that it should be capable of imaging one point of the object field at any time: the entire field is then built up by scanning. This serial imaging has several advantages over the essentially parallel processing of the conventional microscope. In particular, it provides the image in an ideal form for subsequent image processing and display. A typical scanning system and computer interface is shown in Fig. 7.1.
KeywordsFlare Expense Convolution
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- Minsky, M. (1961). U.S. Patent No. 3,013, 467.Google Scholar
- Pawley, J. (1989). Handbook of Biological Confocal Microscopy, I. M.R. Press, University of Wisconsin.Google Scholar
- Wener, H., and Grasserbauer, M., (1991). Analysis of Microelectronic Materials and Devices,Wiley, New York.Google Scholar
- Wilson, T. (ed.) (1990). Confocal Microscopy, Academic Press, New York.Google Scholar
- Wilson, T., and Hamilton, D. K. (1982). J. Microsc. (Oxford) 128 139.Google Scholar
- Wilson, T., and Hewlett, S. J. (1989). Int. Phys. Conf. Ser. No. 98, 629.Google Scholar
- Wilson, T., and Sheppard, C. J. R. (1984). Theory and Practice of Scanning Optical Microscopy, Academic Press, New York.Google Scholar