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Taurine Neurons in Rat Hippocampal Formation are Relatively Inert to Cerebral Ischemia

  • Jang-Yen Wu
  • Chin-Tarng Lin
  • Flemming Fryd Johansen
  • Jie-Wen Liu
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 359)

Abstract

It is well-known that transient cerebral ischemia causes selective loss of neurons in the hippocampus, striatum and cortical layers (14,24). It has been suggested that the difference in nerve cell vulnerability to ischemic damage is probably due to differences in the nature of their transmitters (14,22,27). For instances, GABAergic neurons in the striatum are more susceptible to transient ischemia than the cholinergic neurons (7) and dopaminergic neurons are more sensitive to ischemic damage than the GABAergic neurons in the gerbil brain (26). Since taurine has been demonstrated to have protective effects on the photoreceptor in the cat (12) and on the light-induced disruption of isolated frog rod outer segments (23), we decided to investigate the influence of transient cerebral ischemia on the taurine neurons in rat hippocampus. Taurine neurons were identified immunocytochemically with antibodies against cysteine sulfinic acid decarboxylase, the rate-limiting enzyme of taurine biosynthesis. Cysteine sulfinic acid decarboxylase has previously been used as a specific marker for identification of taurine neurons in the retina (10,17–19), cerebellum (3,4) and hippocampus (10,26,28).

Keywords

Molecular Layer Acid Decarboxylase Neuronal Process Granule Cell Layer Stratum Radiatum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Beneveniste, H., Drejer, J., Schousboe, A., and Diemer, N.H. 1984, Elevation of the extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischemia monitored by intracerebral microdialysis, J.Neurochem., 43:1369–1374.CrossRefGoogle Scholar
  2. 2.
    Berson, E.L., Hayes, K.C., Rabin, A.R., Schmidt, S.Y., and Watson, G. 1976, Retinal degeneration in cat fed casein. II. supplementation with methionine, cysteine or taurine, Invest. Ophthalmol., 15:52–58.Google Scholar
  3. 3.
    Chan-Palay, V., Lin, C.-T., Palay, S., Yamamoto, M., and Wu, J.-Y. 1982, Taurine in the mammalian cerebellum: demonstration by autoradiography with (]3]H)taurine and immunocytochemistry with antibodies against the taurine-synthesizing enzyme, cysteine-sulfinic acid decarboxylase, Proc.Natl.Acad.Sci.USA, 79:2695–2699.CrossRefGoogle Scholar
  4. 4.
    Chan-Palay, V., Palay, S.L., and Wu, J.Y. 1982, Sagittal cerebellar microbands of taurine neurons-immunocytochemical demonstration by using antibodies against the taurine synthesizing enzyme cysteine sulfinic decarboxylase acid, Proc.Natl.Acad.Sci.USA, 79:4221–4225.CrossRefGoogle Scholar
  5. 5.
    Chan-Palay, V., Wu, J.-Y., and Palay, S.L. 1979, Immunocytochemical localization of gamma aminobutyric acid transaminase at cellular and ultrastructure levels, Proc.Natl.Acad.Sci.USA, 76:2067–2071.CrossRefGoogle Scholar
  6. 6.
    Dolara, P., Agresti, A., Giotti, A., and Pasquini, G. 1973, Effect of taurine on calcium kinetics of guinea pig heart, Eur.J.Pharmacol., 24:352–358.CrossRefGoogle Scholar
  7. 7.
    Francis, A. and Pulsinetti, W. 1982, The response of GABAergic and cholinergic neurons to transient cerebral ischemia, Brain Res., 243:271–278.CrossRefGoogle Scholar
  8. 8.
    Franconi, F., Stendardi, I., Matucci, R., Bennardini, F., Baccaro, C., and Giotti, A. 1985, A protective effect of taurine on “hypoxic” and “reoxygenated” guinea pig heart, in: “Taurine: biological actions and clinical perspectives”, Oja, S.S., Ahtee, L., Kontro, P. and Paasonen, M.K., eds., Alan R. Liss, New York, pp. 177–182.Google Scholar
  9. 9.
    Hagberg, H., Lehmann, A., Sandberg, M., Nystrom, B., Jacobsen, I., and Hamberger, A. 1985, Ischemia-induced shift of inhibitory and excitatory amino acids frm intra-and extracellular compartments, J. Cereb.Blood Flow Metab., 5:413–419.CrossRefGoogle Scholar
  10. 10.
    Hanretta, A.T. and Lombardini, J.B. 1987, Is taurine a hypothalamic neurotransmitter?: a model of the differential uptake and compartmentalization of taurine by neuronal and glial cell particles from the rat hypothalamus, Brain Res.Rev., 12:167–201.CrossRefGoogle Scholar
  11. 11.
    Hansen, A.J. 1985, Effect of anoxia on ion distribution in the brain, Physiol.Rev., 65:101–148.Google Scholar
  12. 12.
    Hayes, K.C., Carey, R.E., and Schmidt, S.Y. 1975, Retinal degeneration associated with taurine deficiency in the cat, Science, 188:949–951.CrossRefGoogle Scholar
  13. 13.
    Johansen, F., Lin, C.T., Schousboe, A., and Wu, J.-Y. 1989, An immunocytochemical investigation of L-glutamate decarboxylase in the rat hippocampal formation: The influence of cerebral ischemia, J. Comp.Neurol., 281:40–53.CrossRefGoogle Scholar
  14. 14.
    Jorgensen, M.B. and Diemer, N.H. 1982, Selective neuron loss after cerebral ischemia in the rat: possible role of transmitter glutamate, Acta Neurol.Scand., 66:536–546.CrossRefGoogle Scholar
  15. 15.
    Kuriyama, K., Muramatsu, M., Nakagawa, K., and Kakita, K. 1978, Modulating role of taurine on release of neurotransmitters and calcium transport in excitable tissues, in: “Taurine and Neurological Disorders”, Barbeau, A. and Huxtable,.J., eds., Raven Press, New York, pp. 201–216.Google Scholar
  16. 16.
    Lehmann, A., Hagberg, H., and Hamberger, A. 1984, A role for taurine in the maintenance of homeostasis in the central nervous system during hyperexcitation? Neuroscience Letters, 52:341–346.CrossRefGoogle Scholar
  17. 17.
    Lin, C.-T., Li, H.-Z., and Wu, J.-Y. 1983, Immunocytochemical localization of L-glutamate decarboxylase, gamma-aminobutyric acid transaminase, cysteine sulfinic acid decarboxylase, aspartate aminotransferase and somatostatin in rat retina, Brain Res., 270:273–283.CrossRefGoogle Scholar
  18. 18.
    Lin, C.-T., Song, G.-X., and Wu, J.-Y. 1985, Ultrastructural demonstration of L-glutamate decarboxylase and cysteine sulfinic acid decarboxylase in rat retina by immunocytochemistry, Brain Res., 331:71–80.CrossRefGoogle Scholar
  19. 19.
    Lin, C.-T., Song, G.-X., and Wu, Y.-Y. 1985, Is taurine a neurotransmitter in rabbit retina? Brain Res., 337:293–298.CrossRefGoogle Scholar
  20. 20.
    Lombardini, J.B. 1978, High-affinity transport of taurine in the mammalian central nervous system, in: “Taurine and Neurological Disorders”, Barbeau, A. and Huxtable, R.J., eds., Raven Press, New York, pp. 119–136.Google Scholar
  21. 21.
    Lop’z-Colom’, A.M. 1982, Taurine receptors in CNS membranes: binding studies, in: “Taurine in Nutrition and Neurology”, Huxtable, R.J. and Pasantes-Morales, H., eds., Plenum Press, New York, pp. 293–310.CrossRefGoogle Scholar
  22. 22.
    Nemoto, E.M. 1979, Studies on the pathogenesis of ischemic brain damage and its amelioration by barbiturate therapy, in: “Brain and Heart Infarct”, Zulch, K.J. and Kaufman, W., eds., Springer Verlag, pp. 306-317.Google Scholar
  23. 23.
    Pasantes-Morales, H., Ademe, R.M., and Quesada, O. 1981, Protective effect of taurine on the light-induced disruption of isolated frog rod outer segments, J.Neurochem.Res., 6:337–348.Google Scholar
  24. 24.
    Pulsinelli, W.A. and Brierly, J.B. 1979, A new model of bilateral hemispheric ischemia in the unanesthetized rat, Stroke, 10:267–272.CrossRefGoogle Scholar
  25. 25.
    Schurr, A., Tsen, M.T., and Rigor, B.M. 1986, Protection against hypoxia by taurine — study using the in vitro hippocampal slice, Neurosci.Abstracts, 12:1526.(Abstract).Google Scholar
  26. 26.
    Taber, K.H., Lin, C.T., Liu, J.W., Thalmann, R.H., and Wu, J.Y. 1986, Taurine in hippocampus: localization and postsynaptic action, Brain Res., 386:113–121.CrossRefGoogle Scholar
  27. 27.
    Weinberger, J. and Cohen, C. 1982, Differential effect of ischemia on active uptake of dopamine, GABA, and glutamate, J. Cereb.Blood Flow Metab., 2:265a.Google Scholar
  28. 28.
    Wu, J., Johansen, F.F., Lin, C., and Lui, J. 1987, Taurine system in the normal and ischemic rat hippocampus, in: “The biology of taurine: methods and mechanisms”, Huxtable, R.J., Franconi, F. and Giotti, A., eds., Plenum Press, New York, pp. 265–274.CrossRefGoogle Scholar
  29. 29.
    Wu, J.-Y. 1982, Purification and characterization of cysteic acid and cysteine sulfinic acid decarboxylase and L-glutamate decarboxylase from bovine brain, Proc.Natl.Acad.Sci.USA, 79:4270–4274.CrossRefGoogle Scholar
  30. 30.
    Wu, J.-Y. and Lin, C.-T. 1985, Immunocytochemical techniques, in: “Neuromethods, Vol. 3: Amino Acids”, Boulton, A.A., Baker, G.B. and Wood, J.D., eds., Humana Press, New Jersey, 115–178.Google Scholar
  31. 31.
    Wu, J.-Y., Lin, C.-T., Hwang, B., Wei, S., and Lin, H.S. 1986, Antibodies against enzymes synthesizing amino acid neuro-transmitters, in: “Neurohistochemistry, Modern Methods and Applications”, Panula, P., Paivarinta, H. and Soinila, S., eds., Alan R. Liss, Inc., New York, pp. 21–47.Google Scholar
  32. 32.
    Wu, J.-Y., Tang, X.W., and Tsai, W.H. 1992, Taurine receptor: Kinetic analysis and pharmacological studies, Adv.Exp.Med.Biol., 315:263–268.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Jang-Yen Wu
    • 1
  • Chin-Tarng Lin
    • 3
  • Flemming Fryd Johansen
    • 2
  • Jie-Wen Liu
    • 1
  1. 1.Department of Physiology and Cell BiologyUniversity of KansasLawrenceUSA
  2. 2.Institute of NeuropathologyUniversity of CopenhagenCopenhagenDenmark
  3. 3.Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan

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