Liquid Culture Methods for the Production of Fumonisin

  • Susanne E. Keller
  • Theodore M. Sullivan
Part of the Advances in Experimental medicine and Biology book series (AEMB, volume 392)


Currently, fumonisin B1 is obtained primarily by using solid culture methods. Although fumonisin B1 concentrations obtained in solid culture are typically quite high, subsequent extraction and purification present problems. In addition, current methods utilize complex media which makes analysis of biosynthetic pathways and control mechanisms difficult. Liquid culture methods of production could eliminate many problems associated with production in solid culture. However, in the past, concentrations obtained in liquid culture have been relatively low. In this work, factors affecting the production of fumonisin B1 from a shake flask scale of 100 ml to a fermenter scale of 100 liters were examined. Best results were obtained by using a fed batch method that is nitrogen limited, with pH control. With this method, concentrations in excess of 1000 ppm can be obtained.


Shake Flask Phytic Acid Fusarium Species Catabolite Repression Alternaria Alternata 
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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Susanne E. Keller
    • 1
  • Theodore M. Sullivan
    • 1
  1. 1.Biotechnology Studies BranchUS Food and Drug Administration, National Center for Food Safety and TechnologySummit-ArgoUSA

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