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Antisense Oligonucleotides for in Vivo Studies of Angiotensin Receptors

  • M. Ian Phillips
  • Philipp Ambühl
  • Robert Gyurko
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 396)

Abstract

Synthetic antisense (AS) oligodeoxynucleotides inhibit genetic expression by sequence-specific hybridization to mRNA that renders the mRNA inactive for translation. We have been using AS oligos to lower blood pressure by inhibiting angiotensin receptors and angiotensinogen in freely moving, whole animals. This recent application of antisense technology to in vivo studies, opens a new way of approaching physiological problems with the precision of molecular biology. The possibility of blocking specific gene expression by AS inhibition without multiple, non-specific side effects has potential for therapeutic uses in many diseases. Antisense inhibition is an extremely attractive pharmacological and investigative approach since it offers base-to-base specificity to the target protein and versatility appropriate to the complexity of the genetic code. However, there are a number of issues to be considered before using antisense in any experimental or clinical setting. These include (1) selection of target sequence, (2) the mechanism of cellular uptake, (3) stability of antisense oligos in cells and body fluids, (4) possible intracellular sites of action and (5) effectiveness, in terms of specificity, and duration of action.

Keywords

Cellular Uptake Antisense Oligonucleotide Receptor mRNA Antisense Inhibition Anti Sense Oligonucleotide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • M. Ian Phillips
    • 1
  • Philipp Ambühl
    • 1
  • Robert Gyurko
    • 1
  1. 1.Department of Physiology, College of MedicineUniversity of FloridaGainesvilleUSA

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