Advertisement

AT1-Receptors and Cellular Actions of Angiotensin II in Neuronal Cultures of Stroke Prone-Spontaneously Hypertensive Rat Brain

  • Mohan K. Raizada
  • Di Lu
  • Hong Yang
  • Kan Yu
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 396)

Abstract

AT1 preceptors, its mRNA and cellular actions of angiotensin II (Ang II) have been compared between neuronal cultures of Wistar Kyoto (WKY) and stroke-prone spontaneously hypertensive (SP-SH) rat brains. Bmax for AT1-receptor binding is 2-fold higher and is associated with a parallel increase in the levels of AT1 preceptor mRNA in SP-SH rat brain neurons compared with WKY rat brain neurons. Ang II causes stimulation of both c-fos and norepinephrine transporter (NET) mRNAs in both strains of neurons and this stimulation is also 2–3-fold higher in SP-SH rat brain neurons compared with WKY rat brain neurons. In contrast, Ang II stimulation of PAI-1 mRNA in SP-SH neurons is only 50% that of in WKY rat brain neurons suggesting that SP-SH neurons express a decrease in AT1-receptor coupling with PAI-1 response. These observations demonstrate that SP-SH neurons express AT1-receptor-functions similar to those described for SHR neurons.

Keywords

Losartan Potassium Receptor Gene Expression Spontaneously Hypertensive Sustained Antihypertensive Effect Ofplasminogen Activator 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Saavedra, J.M., 1992, Brain and pituitary angiotensin, Endocrine Rev. 13: 329–380.Google Scholar
  2. 2.
    Timmermans, P.B.M.W.M., Wong, P.C., Chiu, A.T., Herblin, W.F., Benfield, P., Carini, D.J., Lee, R.J., Wexler, R.R., Saye, J.A.M., and Smith, R.D., 1993, Angiotensin II receptors and angiotensin II receptor antagonists, Pharmacol. Rev. 45: 205–251.PubMedGoogle Scholar
  3. 3.
    Wright, J.W., and Harding, J.W., 1994, Brain angiotensin receptor subtypes in the control of physiological and behavioral responses, Neurosci. Biobehavioral Rev. 18: 21–53.CrossRefGoogle Scholar
  4. 4.
    Raizada, M.K., Lu, D., and Sumners, C., 1994, AT1-receptors and angiotensin actions in the brain and neuronal cultures of normotensive and hypertensive rats, In: Current concepts: Tissue renin-angiotensin system as local regulators in Reproductive and Endocrine Organs, (Plenum Press, New York), pp. 331–348.Google Scholar
  5. 5.
    Trippodo, N.C., and Frolich, E.D., 1981, Similarities of genetic (spontaneous) hypertension: Man and rat, Circ. Res. 48: 309–319.PubMedCrossRefGoogle Scholar
  6. 6.
    Johnson, M.I., Ely, D.L., and Turner, M.E., 1992, Genetic divergence between WKY rat and SH rat, Hypertension 19: 425–427.PubMedCrossRefGoogle Scholar
  7. 7.
    Bader, M., Zhao, Y., Sander, M., Lee, M.A., Bachmann, J., Böhn, M., Djavidani, B., Peters, J., Mullins, J.J., and Ganten, D., 1992, Role of tissue renin in the pathophysiology of hypertension in TGR (mREN2)27 rats, Hypertension 19: 681–686.PubMedCrossRefGoogle Scholar
  8. 8.
    Senanayake, P.D., Moriguchi, A., Kumagai, H., Ganten, D., Ferrario, C.M., and Brosnihan, K.B., 1994, Increased expression of angiotensin peptide in the brain of transgenic hypertensive rats, Peptides 15: 919–926.PubMedCrossRefGoogle Scholar
  9. 9.
    Phillips, M.I., Mann, J.F.E., Haebara, H., Hoffman, WE., Dietz, R., Schelling, P., and Ganten, D., 1977, Lowering of hypertension by central saralasin in the absence of plasma renin, Nature 270: 445–447.PubMedCrossRefGoogle Scholar
  10. 10.
    McDonald, W., Wickre, C., Aumann, S., Ban, D., and Meffitt, B., 1980, The sustained antihypertensive effect of chronic ICV infusion of angiotensin antagonist in SH rat, Endocrinol. 107: 1305–1308.CrossRefGoogle Scholar
  11. 11.
    Hutchinson, J.S., Mendelsohn, F.A.O., and Doyle, A.E., 1980, Blood pressure responses of conscious normotensive and spontaneously hypertensive rats to IVT and peripheral administration of Captopril, Hypertension 2: 546–550.PubMedCrossRefGoogle Scholar
  12. 12.
    Okuno, T., Nagahama, S., Lindheimer, M.D., and Oparil, S., 1983, Attenuation of the development of spontaneously hypertension in rats of chronic central administration of Captopril, Hypertension 5: 653–663.PubMedCrossRefGoogle Scholar
  13. 13.
    Gyurko, R., Weilbo, D., and Phillips, M.I., 1993, Antisense inhibition of AT1-receptor mRNA and angiotensinogen mRNA in the brain of spontaneously hypertensive rat reduces hypertension of neurogenic origin, Regul. Pept. 49: 167–174.PubMedCrossRefGoogle Scholar
  14. 14.
    Raizada, M.K., Lu, D., Tang, W., Kurian, P., and Sumners, C., 1993, Increased angiotensin II type 1 receptor gene expression in neuronal cultures from spontaneously hypertensive rats, Endocrinol. 132: 1715–1722.CrossRefGoogle Scholar
  15. 15.
    Sumners, C., and Raizada, M.K., 1993, In: Cellular and molecular biology of renin-angiotensin system, (CRC Press, Boca Raton, FL), pp. 379–411.Google Scholar
  16. 16.
    Sumners, C., Raizada, M.K., Kang, J., Lu, D., and Posner, P., 1994, Receptor-mediated effects of angiotensin II on neurons, Frontiers in Neuroendocrinol. 15: 203–230.CrossRefGoogle Scholar
  17. 17.
    Sumners, C., Tang, W., Zelezna, B., and Raizada, M.K., 1991, Angiotensin II receptor subtypes are coupled with distinct signal transduction mechanisms in neurons and astroglia from rat brain, Proc. Natl. Acad. Sci. USA SS7567-7571.Google Scholar
  18. 18.
    Lu, D., and Raizada, M.K., 1995, Delivery of angiotensin II type 1 receptor antisense inhibits angiotensin action in neurons from hypertensive rat brain, Proc. Natl. Acad. Sci. USA 92: 2914–2918.PubMedCrossRefGoogle Scholar
  19. 19.
    Raizada, M.K., Sumners, C., and Lu, D., 1993, Angiotensin II type 1 receptor mRNA levels in the brains of normotensive and spontaneously hypertensive rats, J. Neurochem. 60: 1949–1952.PubMedCrossRefGoogle Scholar
  20. 20.
    Wang, A.M., Doyle, M.V., and Mark, D.F., 1989, Quantitation of mRNA by the Polymerase chain reaction, Proc. Natl. Acad. Sci. USA 86: 9719–9721.Google Scholar
  21. 21.
    Zelezna, B., Rydzewski, B., Lu, D., Olson, J.A., Shiverick, K.T., Tang, W., Sumners, C., and Raizada, M.K., 1992, Angiotensin II induction of Plasminogen activator inhibitor-1 gene expression in astroglial cells of normotensive and spontaneously hypertensive rat brain, Mol. Endocrinol. 6: 2009–2017.PubMedCrossRefGoogle Scholar
  22. 22.
    Raizada, M.K., and Lu, D., 1995, Angiotensin II (AII) stimulation of norepinephrine transporter (NET) gene expression in neuronal cultures in normotensive and spontaneously hypertensive (SH) rat brain (abstr), FASEB J. part I: A90.Google Scholar
  23. 23.
    Yu, K., and Raizada, M.K., 1995, Angiotensin II (AII) stimulation of Plasminogen activator inhibitor-1 (PAI-1) gene expression in neuronal cultures (NC) of normotensive (WKY) and spontaneously hypertensive (SH) rat brains (abstr), FASEB J. part I: A90.Google Scholar
  24. 24.
    Wilson, K.M., Sumners, C., Hathaway, S., and Fregly, M.J., 1986, Mineralocorticoids modulate central angiotensin II receptors in rats, Brain Res. 382: 87–96.PubMedCrossRefGoogle Scholar
  25. 25.
    Wilson, S.K., Lynch, D.R., and Ladenson, P.W., 1989, Angiotensin II and atrial natriuretic factor-binding sites in various tissues in hypertension: comparative receptor localization and changes in different hypertension models in the rat, Endocrinol 124(6): 2799–2808.CrossRefGoogle Scholar
  26. 26.
    Morishita, R., Higaki, J., Okuniski, H., Nakamura, F., Nagano, M., Mikami, H., Ishii, K., Miyazaki, M., and Ogihara, T., 1993, Role of tissue renin angiotensin system in two-kidney, one-clip hypertensive rats, Am. J. Physiol. 264: F510–F514.PubMedGoogle Scholar
  27. 27.
    Moriguchi, A., Brosnihan, K.B., Kumegai, H., Ganten, D., and Ferrario, C.M., 1994, Mechanisms of hypertension intransgenic rats expressing the mouse Ren-2 gene, Am. J. Physiol. 266 (4Pt2): R1273–1279.PubMedGoogle Scholar
  28. 28.
    Lu, D., Yu, K., and Raizada, M.K., 1995, Retrovirus-mediated transfer of AT1-receptor antisense sequence decreases AT1-receptors and angiotensin II action in astroglial and neuronal cell in primary culture from the brain, Proc. Natl. Acad. Sci. USA 92: 1162–1166.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Mohan K. Raizada
    • 1
  • Di Lu
    • 1
  • Hong Yang
    • 1
  • Kan Yu
    • 1
  1. 1.Department of PhysiologyUniversity of FloridaGainesvilleUSA

Personalised recommendations