Identification of AT1 Receptors on Cultured Astrocytes

  • E. Ann Tallant
  • Debra I. Diz
  • Carlos M. Ferrario
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 396)


It is well established that the brain contains an endogenous renin-angiotensin system (RAS) that participates in the regulation of blood pressure through the control of water intake and sodium appetite, the secretion of ACTH and Vasopressin and the regulation of sympathetic tone (1). While high affinity binding sites for Ang II were initially characterized in brain homogenates (2, 3), the technique of in vitro receptor autoradiography allowed visualization of Ang II binding sites to specific brain regions including the circumventricular organs, hypothalamus, dorsal and ventral medulla, spinal cord and pituitary gland (1, 4, 5). These brain regions correspond to areas at which the peptide exerts its physiological effects (5) or where the responses are inhibited by Ang II receptor antagonists (6).


Inositol Phosphate Scatchard Plot Human Astrocyte Losartan Potassium High Affinity Binding Site 
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  1. 1.
    Ferrario, C.M., Ueno, Y., Diz, D.I. and Barnes, K.L. (1986) in Handbook of Hypertension, Vol.8: Pathophysiology of Hypertension— Regulatory Mechanisms, (eds. A. Zanchetti and R.C. Tarazi), Elsevier Science Publishers B.V. pp 431-54Google Scholar
  2. 2.
    Simonnet, G. and Vincent, J.D. (1982) Neurochem. Int. 4: 149–55PubMedCrossRefGoogle Scholar
  3. 3.
    Bennett, J.P. and Snyder, S.H. (1976) J. Biol. Chem. 251: 7423–30PubMedGoogle Scholar
  4. 4.
    Speth, R.C., Wamsley, J.K., Gehlert, D.R., Chernicky, C.L., Barnes, K.L. and Ferrario, C.M. (1985) Brain Res. 326: 137–43PubMedCrossRefGoogle Scholar
  5. 5.
    Brosnihan, K.B., Diz, D.I., Schiavone, M.T., Averill, D.B. and Ferrario, C.M. (1987) in Brain Peptides and Catecholamines in Cardiovascular Regulation (eds. J.P. Buckley and C.M. Ferrario), Raven Press, New York: pp 313–28Google Scholar
  6. 6.
    Campagnole-Santos, M.J., Diz, D.I., Santos, R.A., Khosla, M.C., Brosnihan, K.B. and Ferrario, C.M. (1988) Hypertension 11 (Supp 1): I–167–71Google Scholar
  7. 7.
    Tallant, E.A., Diz, D.I., Khosla, M.C. and Ferrario, C.M. (1991) Hypertension 17: 1135–43PubMedCrossRefGoogle Scholar
  8. 8.
    Fluharty, S.J. and Reagan, L.P. (1989) J. Neurochem. 52: 1393–400PubMedCrossRefGoogle Scholar
  9. 9.
    Raizada, R.K., Phillips, M.I., Crews, F.T. and Sumners, C. (1987) Proc. Natl. Acad. Sci. USA 84: 4655–9PubMedCrossRefGoogle Scholar
  10. 10.
    Jaiswal, N., Diz, D.I., Tallant, E.A., Khosla, M.C. and Ferrario, C.M. (1991) Am. J. Physiol. Regul. Integr. Comp. Physiol. 260: R1000–6Google Scholar
  11. 11.
    Jaiswal, N., Tallant, E.A., Diz, D.I., Khosla, M.C. and Ferrario, C.M. (1991) Hypertension 17: 1115–20PubMedCrossRefGoogle Scholar
  12. 12.
    Speth, R.C. and Harik, S.I. (1985) Proc. Natl. Acad. Sci. USA 82: 6340–3PubMedCrossRefGoogle Scholar
  13. 13.
    Guillot, F.L. and Audus, K.L. (1991) Peptides 12: 535–40PubMedCrossRefGoogle Scholar
  14. 14.
    Thomas, W.G., Greenland, K.J., Shinkel, T.A. and Sernia, C. (1992) Brain Res. 588: 191–200PubMedCrossRefGoogle Scholar
  15. 15.
    Milsted, A., Barna, B.P., Ransohoff, R.M., Brosnihan, K.B. and Ferrario, C.M. (1990) Proc. Natl. Acad. Sci. USA 87: 5720–3PubMedCrossRefGoogle Scholar
  16. 16.
    Deschepper, C.F., Bouhnik, J. and Ganong, W.F. (1986) Brain Res. 374: 195–8PubMedCrossRefGoogle Scholar
  17. 17.
    Stornetta, R.L., Hawelu-Johnson, C.L., Guyenet, P.G. and Lynch, K.R. (1988) Science 242: 1444–6PubMedCrossRefGoogle Scholar
  18. 18.
    Gyurko, R., Wielbo, D. and Phillips, M.I. (1993) Regul. Pept. 49: 167–74PubMedCrossRefGoogle Scholar
  19. 19.
    Printz, M.P., Jennings, C., Healy, D.P. and Kalter, V. (1986) J. Cardiovasc. Pharmacol. 8(Suppl 10): S62–8PubMedCrossRefGoogle Scholar
  20. 20.
    Hosli, E. and Hosli, L. (1989) Neuroscience 31: 463–70PubMedCrossRefGoogle Scholar
  21. 21.
    Bottari, S.P., Obermüller, N., Bogdal, Y., Zahs, K.R. and Deschepper, C.F. (1992) Brain Res. 585: 372–6PubMedCrossRefGoogle Scholar
  22. 22.
    Barna, B.P., Chou, S.M., Jacobs, B., Ransohoff, R.M., Hahn, J.F. and Bay, J.W. (1985) J. Neuroimmunol. 10: 151–8PubMedCrossRefGoogle Scholar
  23. 23.
    Estes, M.L., Ransohoff, R.M., McMahon, J.T., Jacobs, B.S. and Barna, B.P. (1991) J. Neurosci. Res. 27: 697–705CrossRefGoogle Scholar
  24. 24.
    Smith, J.B. (1986) Am. J. Physiol. 250: F759–69PubMedGoogle Scholar
  25. 25.
    Campanile, C.P., Crane, J.K., Peach, M.J. and Garrison, J.C. (1982) J. Biol. Chem. 257: 4951–8PubMedGoogle Scholar
  26. 26.
    Johnson, R.M. and Garrison, J.C. (1987) J. Biol. Chem. 262: 17285–93PubMedGoogle Scholar
  27. 27.
    Tallant, E.A., Jaiswal, N., Diz, D.I. and Ferrario, C.M. (1991) Hypertension 18: 32–9PubMedCrossRefGoogle Scholar
  28. 28.
    Jaiswal, N., Diz, D.I., Tallant, E.A., Khosla, M.C. and Ferrario, C.M. (1991) Am. J. Hypertens. 4: 228–33PubMedCrossRefGoogle Scholar
  29. 29.
    Cole, R. and de Vellis, J. (1989). in A Dissection and Tissue Culture Manual of the Nervous System (eds. A. Shahar, J. de Vellis, A. Vernadakis and B. Haber), Alan R. Liss, Inc. New York: pp 121–33Google Scholar
  30. 30.
    Berridge, M.J. (1983) Biochem. J. 212: 849–58PubMedGoogle Scholar
  31. 31.
    Sumners, C., Tang, W., Zelezna, B. and Raizada, M.K. (1991) Proc. Natl. Acad. Sci. USA 88: 7561–11CrossRefGoogle Scholar
  32. 32.
    Sumners, C., Meyers, L.M., Kalberg, C.J. and Raizada, M.K. (1990) Prog. Neurobiol. 34: 355–85PubMedCrossRefGoogle Scholar
  33. 33.
    Tsutsumi, K. and Saavedra, J.M. (1991) Am. J. Physiol. Regul. Integr. Comp. Physiol. 261: R209–16Google Scholar
  34. 34.
    Khayyall, M., MacGregor, J., Brown, J.J., Lever, A.F. and Robertson, J.I.S. (1973) Clin. Sci. 44: 87–90PubMedGoogle Scholar
  35. 35.
    Kohara, K., Brosnihan, K.B., Ferrario, C.M. and Milsted, A. (1992) Am. J. Physiol. 262: E651–7PubMedGoogle Scholar
  36. 36.
    Nishimura, M., Milsted, A., Block, C.H., Brosnihan, K.B. and, Ferrario, C.M. (1992) Hypertension 20: 158–67PubMedCrossRefGoogle Scholar
  37. 37.
    Chiu, E.K. and Richardson, J.S. (1983) J. Gen. Pharmacol. 14: 553–63CrossRefGoogle Scholar
  38. 38.
    Brooks, D.P., Share, L. and Crofton, J.T. (1986) Endocrinology 118: 1716–22PubMedCrossRefGoogle Scholar
  39. 39.
    Inoui, M., Crofton, J.T., Toba, K. and Share, L. (1990). FASEB J. 4: A683Google Scholar
  40. 40.
    White, R.P. and Hagen, A.A. (1982) Pharmacol. Ther. 18: 313–31PubMedCrossRefGoogle Scholar
  41. 41.
    Wahl, A., Unterberg, A., Baethmann, A. and Schilling, L. (1988) J. Cereb. Blood Flow Metab. 8: 621–34PubMedCrossRefGoogle Scholar
  42. 42.
    Palmer, G.C. (1986) Neurosci. Biobehav. Rev. 10: 79–101PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • E. Ann Tallant
    • 1
  • Debra I. Diz
    • 1
  • Carlos M. Ferrario
    • 1
  1. 1.Hypertension CenterBowman Gray School of MedicineWinston-SalemUSA

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