Antisense RNA in Plants: A Tool for Analysis and Suppression of Gene Function

  • Martin Tabler
Chapter
Part of the NATO ASI Series book series (NSSA, volume 253)

Abstract

Expression of antisense genes has become a widely applied technique in plant biotechnology for creating “phenotypic mutants” in which the endogenous target gene is specifically suppressed. The antisense technology is used for various purposes such as silencing or ablating undesired genes. By creating “dominant negative mutants”, gene function can also be assigned to a particular cloned cDNA. Despite the rapidly increasing number of successful applications of antisense genes in plants, the molecular mechanisms in which complementary RNAs down-regulate gene expression is not fully understood.

Keywords

Starch Assimilation Photosynthesis Flavonoid Cytosol 

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Martin Tabler
    • 1
  1. 1.Institute of Molecular Biology and BiotechnologyFoundation for Research and TechnologyHeraklionGreece

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