Skip to main content
SpringerLink
Log in
Book cover

Esterases, Lipases, and Phospholipases pp 253–261Cite as

Detection of Phospholipases A2 in Serum in Acute Pancreatitis

  • Chapter

  • 91 Accesses

Part of the book series: NATO ASI Series ((NSSA,volume 266))

Abstract

Phospholipase A2 (PLA2, E.C. 3.1.1.4) hydrolyzes phospholipids, e.g. phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol into corresponding lysocompounds. PLA2 is the rate-limiting enzyme in the formation of eicosanoids, which are mediators of inflammation. It is assumed that PLA2 plays an important role in the pathology of various diseases, such as acute pancreatitis, septic shock and multiple injuries [1, 2, 3, 4, 5].

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Nevalainen T.J. The role of phospholipase A2 in acute pancreatitis. Scand J Gastroenterol 15; 1980: 641–650.

    Article  PubMed  CAS  Google Scholar 

  2. Nevalainen T.J. Phospholipase A2 in acute pancreatitis. Scand J Gastroenterol 23; 1988: 897–904.

    Article  PubMed  CAS  Google Scholar 

  3. Vadas P. and Pruzanski W. Role of secretory phospholipase A2 in the pathobiology of disease. Lab Invest 55; 1986: 391–404.

    PubMed  CAS  Google Scholar 

  4. Uhl W., Büchler M., Nevalainen T.J., Deller A. and Beger H.G. Serum phospholipase A2 in patients with multiple injuries. J Trauma 30; 1990: 1285–1290.

    Article  PubMed  CAS  Google Scholar 

  5. Pruzanski W., and Vadas P. Phospholipase A2–a mediator between proximal and distal effectors of inflammation. Immunol Today 12; 1991: 143–146.

    PubMed  CAS  Google Scholar 

  6. Creutzfeldt W. and Schmidt H. Aetilogy and pathogenesis of pancreatitis. Current concepts. Scand J Gastroenterol (Suppl) 6; 1970: 47–62.

    CAS  Google Scholar 

  7. Heinrikson R.L., Krueger E.T. and Keim P.S. Amino acid sequence of phospholipase A2 from the venom of Crotalus adamanteus. A new classification of phospholipases A2 based upon structural determinants. J Biol Chem 252; 1977: 4913–4921.

    PubMed  CAS  Google Scholar 

  8. van den Bosch H., Aarsman A.J., van Schaik R.H.N., Schalkwijk C.H., Nejs F.W. and Sturk A. Structural and enzymological properties of cellular phospholipase A2. Biochem Soc Trans 18; 1990: 781–786.

    PubMed  Google Scholar 

  9. Reynolds L.J., Washburn W.N., Deems R.A. and Dennis E.A. Assay strategies and methods for phospholipases. Methods Enzymol 197; 1991: 3–23.

    Article  PubMed  CAS  Google Scholar 

  10. Patriarca P., Beckerdite S. and Elsbach P. Phospholipases and phospholipid turnover in Escherichia coli spheroplasts. Biochim Biophys Acta 260; 1972: 593–600.

    Article  PubMed  CAS  Google Scholar 

  11. Shakir K.M.M. Phospholipase A2 activity of post-heparin plasma; a rapid and sensitive assay and partial characterization. Anal Biochem 114; 1981: 64–70.

    Article  PubMed  CAS  Google Scholar 

  12. Schädlich H.R., Büchler M. and Beger H.G. Improved method for the determination of phospholipase A2 catalytic activity concentration in human serum and ascites. J Clin Chem Clin Biochem 25; 1987: 505–509.

    PubMed  Google Scholar 

  13. Nishijima J., Okamoto M., Ogawa M., Kasaki G. and Yamano T. Purification and characterization of human pancreatic phospholipase A2 in development of a radioimmunoassay. J Biochem 94; 1983: 137–147.

    PubMed  CAS  Google Scholar 

  14. Eskola J.U., Nevalainen T.J. and Lövgren T.N.-E. Time-resolved fluoroimmunoassay of human pancreatic phospholipase A2. Clin Chem 29; 1983: 1777–1780.

    PubMed  CAS  Google Scholar 

  15. Oka Y., Ogawa M., Matsuda Y., Murata A., Nishijima J., Miyauchi K., Uda K., Yasuda T. and Mori T. Serum immunoreactive pancreatic phospholipase A2 in patients with various malignant tumours. Enzyme 43; 1990: 80–88.

    PubMed  CAS  Google Scholar 

  16. Santavuori S.A., Kortesuo P.T., Eskola J.U. and Nevalainen T.J. Application of a new monoclonal antibody for time-resolved fluoroimmunoassay of human pancreatic phospholipase A2. Eur J Clin Chem Clin Biochem 29; 1991: 819–826.

    PubMed  CAS  Google Scholar 

  17. Matsuda Y., Ogawa M., Sakamoto K., Yamashita S., Kanda A., Kohno M., Yoshida N., Nishijima J., Murata A. and Mori T. Development of a radioimmunoassay for human group II phospholipase A2 and demonstration of postoperative elevation. Enzyme 45; 1991: 200–208.

    PubMed  CAS  Google Scholar 

  18. Nevalainen T.J., Kortesuo P.T., Rintala E. and Märki F. Immunochemical detection of group I and group II phospholipases A2 in human serum. Clin Chem 38; 1992: 1824–1829.

    PubMed  CAS  Google Scholar 

  19. Ogawa M., Arakawa H., Yamashita S., Sakamoto K. and Ikei S. Postoperative elevations of serum interleukin 6 and group II phospholipase A2: group II phospholipase A2 in serum is an acute phase reactant. Res Commun Chem Pathol Pharmacol 75; 1992: 109–115.

    PubMed  CAS  Google Scholar 

  20. Nevalainen T.J., Grönroos J.M. and Kortesuo P.T. Pancreatic and synovial-type phospholipase A2 in serum in severe acute pancreatitis. Gut 34; 1993: 1133–1136.

    Article  PubMed  CAS  Google Scholar 

  21. Rintala E.M. and Nevalainen T.J. Synovial-type (group II) phospholipase A2 in serum of febrile patients with a haematological malignancy. Eur J Haematol 50; 1992: 11–16.

    Article  Google Scholar 

  22. Pulkkinen M.O., Kivikoski A._.id Nevalainen T.J. Group I and group II phospholipases A2 in serum during normal and pathological pregnancy. Gynecol Obstet Invest,in press 1993.

    Google Scholar 

  23. Schröder T., Kivilaakso E., Kinnunen P.K.J. and Lempinen M. Serum phospholipase A2 in human acute pancreatitis. Scand J Gastroenterol 15; 1980: 633–636.

    Article  PubMed  Google Scholar 

  24. Nevalainen T.J. and Haapanen T.J. Distribution of pancreatic (group I) and synovial-type (group II) phospholipases A2 in human tissues. Inflammation 17;1993:453464.

    Google Scholar 

  25. Schröder T. The effect of early pancreatectomy and peritoneal lavage on the development of experimental hemorrhagic pancreatitis (EHP) in pigs. Scand J Gastroenterol 17; 1982: 167–171.

    Article  PubMed  Google Scholar 

  26. Eskola J.U., Nevalainen T.J. and Kortesuo P.T. Immunoreactive pancreatic phospholipase A2 and catalytically active phospholipase A2 in serum from patients with acute pancreatitis. Clin Chem 34; 1988: 1052–1054.

    PubMed  CAS  Google Scholar 

  27. Büchler M., Malfertheiner P., Schädlich H., Nevalainen T.J., Friess H. and Beger H.G. Role of phospholipase A2 in human acute pancreatitis. Gastroenterology 97; 1989: 1521–1526.

    PubMed  Google Scholar 

  28. Crowl R.M., Stoller T.J., Conroy R.R. and Stoner C.R. Induction of Phospholipase A2 gene expression in human hepatoma cells by mediators of acute phase response. J Biol Chem 266; 1991: 2647–2651.

    PubMed  CAS  Google Scholar 

  29. Opie E.L. and Meakins J.C. Data concerning the etiology and pathology of hemorrhagic necrosis of the pancreas (acute hemorrhagic pancreatitis). J Exp Med 11;1909:561–578.

    Google Scholar 

  30. Schmidt H. and Creutzfeldt W. The possible role of phospholipase A in the pathogenesis of acute pancreatitis. Scand J Gastroenterol 4; 1969: 39–48.

    Article  PubMed  CAS  Google Scholar 

  31. Aho H.J., Nevalainen T.J., Lindberg R.L.P. and Aho A.J. Experimental pancreatitis in the rat. The role of phospholipase A in sodium taurocholate-induced acute haemorrhagic pancreatitis. Scand J Gastroenterol 15; 1980: 1027–1031.

    Article  PubMed  CAS  Google Scholar 

  32. Aho H.J. and Nevalainen T.J. Experimental pancreatitis in the rat. Light and electron microscopical observations on early pancreatic lesions induced by intraductal injection of trypsin, phospholipase A2, lysolecithin and non-ionic detergent. Virchows Arch (Cell Pathol) 40; 1982: 347–356.

    Google Scholar 

  33. Wilson H.A., Askari A.D., Neiderhiser D.H., Johnson A.M., Andrews B.S. and Hoskins L.C. Pancreatitis with arthropathy and subcutaneous fat necrosis. Arthritis Rheum 26; 1983: 121–126.

    Article  PubMed  CAS  Google Scholar 

  34. Goke B., Meyer T., Loth H., Adler G. and Arnold R. Characterization of phospholipase A2 activity in aspirates of human pancreatic pseudocysts after isolation by reversed-phase high performance liquid chromatography. Klin Wochenschr 67 (3); 1989: 110–112.

    Article  Google Scholar 

  35. Grönroos J.M. and Nevalainen T.J. Increased concentration of synovial-type phospholipase A2 in serum and pulmonary and renal complications in acute pancreatitis. Digestion 52; 1992: 232–236.

    Article  PubMed  Google Scholar 

  36. Yasuoka A. and Ogawa R. Changes in pancreatic lecithinase A and its significance during shock. Jap J Anesthesiol 28; 1979: 681–688.

    Google Scholar 

  37. Holm B.A., Keicher L., Liu M.Y., Sokolowski J. and Enhorning G. Inhibition of pulmonary surfactant function by phospholipases. J Appl Physiol 71;1991:317321.

    Google Scholar 

  38. Bird N.C., Goodman A.J. and Johnsson A.G. Serum phospholipase A2 activity in acute pancreatitis: an early guide to severity. Br J Surg 76; 1989: 731–732.

    Article  PubMed  CAS  Google Scholar 

  39. Hietaranta A.J., Aho H.J., Grönroos J.M., Hua Z.Y. and Nevalainen T.J. Pancreatic phospholipase A2 in proximal tubules of rat kidney in experimental acute pancreatitis and after intravenous injection of the enzyme. Pancreas 7;1992:326333.

    Google Scholar 

  40. Grönroos J.M., Hietaranta A.J. and Nevalainen T.J. Renal tubular cell injury and serum phospholipase A2 activity in human acute pancreatitis. Br J Surg 79;1992:800801.

    Google Scholar 

  41. Matthys E., Yogendra P., Kreisberg J., Stewart J.H. and Venkatachalam M. Lipid alterations induced by renal ischaemia: pathogenic factor in membrane damage. Kidney Int 26; 1984: 153–161.

    Article  PubMed  CAS  Google Scholar 

  42. Kreutner W. and Siegal M.I. Biology of leukotrienes. Ann Rep Med Chem 19; 1984: 24 1251.

    Google Scholar 

  43. Nguyen V.O.D., Cieslinski D.A. and Humes H.D. Importance of adenosine triphosphate in phospholipase A2-induced rabbit renal proximal tubular cell injury. J Clin Invest 82; 1988: 1098–1105.

    Article  PubMed  CAS  Google Scholar 

  44. Humes H.D., Nguyen V.O.D., Cieslinski D.A. and Messana J.M. The role of free fatty acids in hypoxia-induced injury to renal proximal tubule cells. Am J Physiol 256; 1989: F688 - F696.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, University of Turku, Turku, Finland

    Timo J. Nevalainen

  2. Department of Surgery, University of Turku, Turku, Finland

    Juha M. Grönroos

Authors
  1. Timo J. Nevalainen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juha M. Grönroos
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Manchester, Manchester, UK

    M. I. Mackness

  2. University of Bordeaux II, Bordeaux, France

    M. Clerc

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer Science+Business Media New York

About this chapter

Cite this chapter

Nevalainen, T.J., Grönroos, J.M. (1994). Detection of Phospholipases A2 in Serum in Acute Pancreatitis. In: Mackness, M.I., Clerc, M. (eds) Esterases, Lipases, and Phospholipases. NATO ASI Series, vol 266. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0993-0_30

Download citation

  • DOI: https://doi.org/10.1007/978-1-4899-0993-0_30

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-0995-4

  • Online ISBN: 978-1-4899-0993-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation