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Molecular Studies of Antigens in HAP Organisms

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Haemophilus, Actinobacillus, and Pasteurella

Abstract

The use of recombinant DNA technology to study the HAP organisms has greatly improved our ability to characterize the many potential virulence factors of these bacteria. Instead of relying on conventional identification and purification methods based on biochemical, biophysical or immunological techniques, the genes coding for the virulence factors could be isolated for more detailed studies. After isolation and characterization of the genes, the recombinant antigens could be expressed for detailed biochemical and immunological studies. Further, large scale production of the recombinant antigens is also possible by high level expression in a heterologous system. These recombinant antigens could be used in vaccine trial and challenge experiments to investigate their protective capabilities. As a result of these molecular approaches, we now have a better understanding of some of the virulence factors of the HAP organisms. Some of these characterized antigens are being used as potential candidates for the development of an efficacious vaccine against these opportunistic pathogens in animals. In addition to studying the virulence antigens of the bacteria, the accumulation of molecular data is beginning to give us a picture into the biology, genetics and evolution of these bacteria.

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  1. Department of Microbiology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1

    R. Y. C. Lo

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  1. Moredun Research Institute, Edinburgh, Scotland

    W. Donachie , F. A. Lainson  & J. C. Hodgson ,  & 

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Lo, R.Y.C. (1995). Molecular Studies of Antigens in HAP Organisms. In: Donachie, W., Lainson, F.A., Hodgson, J.C. (eds) Haemophilus, Actinobacillus, and Pasteurella. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0978-7_11

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