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Morphological and Physiological Studies of Tectal and Pretectal Neurons in the Frog

  • Nobuyoshi Matsumoto

Abstract

Intracellular recording and staining techniques were applied to visual neurons in frog’s optic tectum and pretectum to study receptive fields, response characteristics, cellular morphologies, axonal projections, and synaptic organizations. In the first experiment, response properties of labeled neurons, classified according to extracellular studies, could be correlated with morphology. Class T5.1 neurons were identified as large ganglionic neurons in layer 8 or 7, and pear-shaped neurons in layer 8 or the top of 6. No obvious relationship was found between physiological and morphological properties. Class T5.2 neurons were located on top of layer 6 and resembled the pyramidal type; an axon could be traced to the medulla oblongata. Class T5.3 neurons were identified as large ganglionic neurons in layer 8. Neurons responding predominantly by IPSPs could be identified as pear-shaped neurons in layers 2, 4, or 6. In the second experiment, axonal projections of neurons in the pretectal caudal thalamus were examined Some neurons from the Lpd (lateral posterodorsal) nucleus projected bilaterally to both optic lobes or to the contralateral tectum; from P (posterocentral) nucleus neurons projected toward the medulla oblongata. To electrical stimulation of the optic tract, the former neurons exhibited an EPSP and the latter an EPSP followed by IPSP. In the third experiment, a pulse-triggered averaging technique was applied to investigate retino-tectal connections. Synaptic organizations between retinal on-off fibers and tectal E-E type neurons (EPSP at on and at off of diffuse light) and EI-EI type neurons (EPSP and IPSP at on and at off of diffuse light), respectively, were examined. It was found that tectal neurons of these types are monosynaptically excited by on-off retinal fibers.

Keywords

Medulla Oblongata Optic Tectum Diffuse Light Axonal Projection Bufo Bufo 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Antal M, Matsumoto N, Székely G (1986) Tectal neurons of the frog: intracellular recording and labeling with cobalt electrodes. J Comp Neurol 246: 238–253PubMedCrossRefGoogle Scholar
  2. Arbib MA (1982) Modelling neuronal mechanisms of visuomotor coordination in frog and toad. In: Amari S, Arbib MA (eds) Competition and cooperation in neuronal nets Springer-Verlag, Berlin Heidelberg New York, pp 342–370CrossRefGoogle Scholar
  3. Brookhart JM, Fadiga E (1960) Potential field initiated during monosynaptic activation of frog motoneurons. J Physiol 150: 633–655PubMedGoogle Scholar
  4. Ewert J-P (1968) Der Einfluß von Zwischenhirndefekten auf die Visuomotorik im Beute-and Fluchtverhalten der Erdkröte (Bufo bufo L). Z Verg! Physiol 61: 41–70Google Scholar
  5. Ewert J-P (1971) Single unit response of the toad (Bufo americanus) caudal thalamus to visual objects. Z Verg Physiol 74: 81–102CrossRefGoogle Scholar
  6. Ewert J-P (1984) Tectal mechanisms that underlie prey-catching and avoidance behaviors in toads. In: Vanegas H (ed) Comparative neurology of the optic tectum. Plenum Press, New York London, pp 247–399Google Scholar
  7. Ewert J-P, Hock FJ, Wietersheim A v (1974) Thalamus/Praetectum/Tectum: retinale Topographie and physiologische Interaktionen bei der Kröte (Bufo bufo). J Comp Physiol 92: 343–356CrossRefGoogle Scholar
  8. Ewert J-P, Matsumoto N, Schwippert WW (1985) Morphological identification of prey-selective neurons in the grass frog’s optic tectum. Naturwissenschaften 72: 661–662PubMedCrossRefGoogle Scholar
  9. Görcs T, Antal M, Olah E, Székely G (1979) An improved cobalt labelling technique with complex compounds. Acta Biol Acad Sci Hung 30: 79–86PubMedGoogle Scholar
  10. Grösser O-J, Grüsser-Cornehls U (1976) Neurophysiology of the anuran visual system. In: Llinds R, Precht W (eds) Frog neurobiology. Springer-Verlag, Berlin Heidelberg New York, pp 298–385Google Scholar
  11. Hardy O, Leresche N, Jassik-Geschenfeld (1985) Morphology and laminar distribution of electrophysiologically identified cells in the pigeon’s optic tectum: an intracellular study. J Comp Neurol 233: 390–404Google Scholar
  12. Lara R, Cervantes F, Arbib MA (1982) Two-dimensional model of retinal-tectal-pretectal interactions for the control of prey-predator recognition and size preference in amphibia. In: Amari S, Arbib MA (eds) Competition and cooperation in neural nets Springer-Verlag, Berlin Heidelberg New York, pp 371–393Google Scholar
  13. Lâzdr G (1969) Efferent pathways of the optic tectum in the frog. Acta Biol Acad Sci Hung 19: 325–334Google Scholar
  14. Lazdr G (1984) Structure and connection of the frog optic tectum. In: Vanegas H (ed) Comparative neurology of the optic tectum. Plenum Press, New York London, pp 185–210Google Scholar
  15. Lr G, Toth P, Csank G, Kicliter E (1983) Morphology and location of the tectal projection neurons in frogs: a study with HRP and cobalt-filling. J Comp Neurol 215: 108–120CrossRefGoogle Scholar
  16. Lettvin JY, Maturana HR, McCulloch WS, Pitts WH (1959) What the frog’s eye tells the frog’s brain. Proc Inst Radio Engin 47: 1940–1951Google Scholar
  17. Matsumoto N, Bando T (1978) Intracellular recordings of tectal cells of the frog. Proc Jap Acad 54B: 386–390CrossRefGoogle Scholar
  18. Matsumoto N, Bando T (1980) Excitatory synaptic potentials and morphological classification of tectal neurons of the frog. Brain Res 192: 39–48PubMedCrossRefGoogle Scholar
  19. Matsumoto N, Schwippert WW, Ewert J-P (1986) Intracellular activity of morphologically identified neurons of the grass frog’s optic tectum in response to moving configurational visual stimuli. J Comp Physio! 159: 721–739CrossRefGoogle Scholar
  20. Nagano K, Li QL, Tamada A, Matsumoto N (1988) An analysis of postsynaptic potentials of tectal neurons of the frog. correlation with impulses recorded from the terminals of retino-tectal afferents. P_xp Brain Res70: 429–432Google Scholar
  21. Perkel DH, Gerstein GL, Moor GP (1967) Neuronal spike trains. II: Simultaneous spike trains. Biophysical J 7: 419–440Google Scholar
  22. Rubinson K (1968) Projections of the tectum opticum of the frog. Brain Behav Evol 1: 529–561CrossRefGoogle Scholar
  23. Satou M, Ewert J-P (1985) The antidromic activation of tectal neurons by electrical stimuli applied to the caudal medulla oblongata in the toad Bufo bufo (L). JComp Physiol 157: 739–748CrossRefGoogle Scholar
  24. Schürg-Pfeiffer E, Ewert J-P (1981) Investigation of neurons involved in the analysis of Gestalt prey features in the frog Rana temporaries. J Comp Physiol 141: 139–158CrossRefGoogle Scholar
  25. Székely G, Ldzâr G (1976) Cellular and synaptic architecture of the optic tectum. In: Llinâs R, Precht W (eds) Frog neurobiology. Springer-Verlag, Berlin Heidelberg New York, pp 407–437CrossRefGoogle Scholar
  26. Tanaka K (1983) Cross-correlation analysis of geniculostriate neuronal relationships in cats. J Neurophysiol 49: 1303–1318PubMedGoogle Scholar
  27. Toyama K, Kimura M, Tanaka K (1981) Cross-correlation analysis of interneuronal connectivity in cat visual cortex. JNeurophysiol 46: 191–201Google Scholar
  28. Tsai HJ, Ewert J-P (1988) Influence of stationary and moving background textures of the response of visual neurons in toads (Bufo bufo). Brain Behav Evol (in press)Google Scholar
  29. Urban L, Székely G (1983) Intracellular staining of motoneurons with cobalt complex compounds in the frog. J Neurobio 114: 157–161Google Scholar
  30. Weerasuriya A, Ewert J-P (1981) Prey-selective neurons in the toad’s optic tectum and sensorimotor interfacing: HRP studies and recording experiments. J Comp Physiol 144: 429–434CrossRefGoogle Scholar
  31. Weerasuriya A, Ewert J-P (1983) Afferents of some dorsal retino-recipient areas of the brain of Bufo bufa Soc Neurosci Abstr 9: 536Google Scholar
  32. Wilczynski W, Northcutt R (1977) Afferents to the optic tectum of the leopard frog: an HRP study. J Comp Neuro 1173: 219–229CrossRefGoogle Scholar
  33. Witpaard J, ter Keurs HEDL (1975) A reclassification of retinal ganglion cells in the frog, based upon tectal endings and response properties. Vision Res 15: 1933–1938CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Nobuyoshi Matsumoto
    • 1
  1. 1.Department of Biophysical Engineering Faculty of Engineering ScienceOsaka UniversityToyonaka, Osaka 560Japan

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