Platelet-Activating Factor (PAF) Receptor in Brain and Signal Transduction in Neurons

  • Tian-Li Yue
  • Paul G. Lysko
  • Giora Feuerstein
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)


Specific, reversible and saturable binding sites for [3H]-PAF were observed in gerbil brain. Scatchard analysis revealed the existence of two apparent classes of binding sites with Kd1=3.7 nM and Kd2=20.4 nM. The maximal density of binding sites was found in midbrain and hippocampus (Domingo et al., 1988). The existence of specific binding sites for PAF was also shown in rat hypothalamus (Junier et al., 1988) and synaptosomes and microsomal fractions of rat cerebral cortex (Marcheselli et al., 1990).


Global Brain Ischemia Gerbil Brain Bilateral Carotid Artery Occlusion Calcium Channel Blocker Nifedipine Rabbit Spinal Cord Ischemia 
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  1. Bielenberg GW, 1988, “Pharmacology of Cerebral Ischemia,” Krieg!stein, J., ed., CRC Press, Boca Raton.Google Scholar
  2. Domingo, M.T., Spinnewyn, B., Chabrier, P.E., and Braquet, P., 1988, Presence of specific binding sites for platelet-activating factor (PAF) in brain. Biochem. Biophys. Res. Commun. 151: 730–736.Google Scholar
  3. Frerichs, K.U., Lindsberg, P.J., Hallenbeck, J.M., and Feuerstein, G., 1990, Platelet-activating factor and progressive brain damage following brain injury, J. Neurosurg. 73: 223–233.PubMedCrossRefGoogle Scholar
  4. Junier, M.P., Tiberghien, C., Rougeot, C., Fafeur, V. and Dray, F., 1988, Inhibitory effect of platelet-activating factor ( PAF) on luteinizing hormone-releasing hormone and somatostatin release from rat median eminence in vitro correlated with the characterization of specific PAF receptor sites in rat hypothalamus. Endocrinology 123: 72–80.Google Scholar
  5. Kochanek, P.M., Dutka, A.J., Kumaroo, K.K. and Hallenbeck, J.M., 1987, Platelet-activating factor receptor blockade enhances recovery after multifocal brain ischemia. Life Sci. 41: 2639–2644.PubMedCrossRefGoogle Scholar
  6. Kochanek, P.M., Schoettle, R., Uhl, M., Magargee, M.J. and Nemoto, E., 1991, Platelet-activating factor antagonists do not attenuate delayed posttraumatic cerebral edema in rats. J. Neurotrauma 8: 19–25.PubMedCrossRefGoogle Scholar
  7. Lindsberg, P.J., Yue, T-L., Frerichs, K.U., Hallenbeck, J.M. and Feuerstein, G., 1990, Evidence of Platelet-activating factor ( PAF) as a novel mediator in experimental stroke in rabbits. Stroke 21: 1452–1457.Google Scholar
  8. Marcheselli, V.L., Rossowska, M.J., Domingo, M.T., Braquet, P. and Bazan, N.G., 1991, Distinct platelet-activating factor binding sites in synaptic endings and in intracellular membranes of rat cerebral cortex. J. Biol. Chem. (In press).Google Scholar
  9. Oberpichler, H., Sauer, D., Robberg, C., Mennel, H.D. and Krieglstein, J., 1990, PAF antagonist ginkgolide B reduces postischemic neuronal damage in rat brain hippocampus. J. Cereb. Blood Flow Metab. 10: 133–135.Google Scholar
  10. Panetta, T., Marcheselli, V.L., Braquet, P., Spinnewyn, B. and Bazan, N.G., 1987, Effects of a platelet-activating factor antagonist (BN52021) on free fatty acids, diacylglycerols, polyphosphoinositides and blood flow in the gerbil brain: inhibition of ischemia-reperfusion induced cerebral injury. Biochem. Biophys. Res. Commun. 149: 580–587.Google Scholar
  11. Spinnewyn, B., Blaret, N., Clostre, F., Bazan, N. and Braquet, P., 1987, Involvement of platelet-activating factor ( PAF) in cerebral post-ischemic phase in mongolian gerbils. Prostaglandins 34: 337–349.Google Scholar
  12. Yue, T-L., Gleason, M.M., Hallenbeck, J. and Feuerstein, G., 1991, Characterization of platelet-activating factor-induced elevation of cytosolic free-calcium level in neurohybrid NCB-20 cells. Neurosci. 44: 177–185.CrossRefGoogle Scholar
  13. Yue, T-L., Gleason, M.M., Gu, J-L., Lysko, P.G., Hallenbeck, J., and Feuerstein, G., 1991, Platelet-activating factor (PAF) receptor-mediated calcium mobilization and phosphoinositide turnover in neurohybrid NG10815 cells: studies with BN50739, a new PAF antagonist. J. Pharmacol. Exp. Ther. 257: 374–381.Google Scholar
  14. Yue, T-L., Rabinovici, R., Farhat, M. and Feuerstein, G., 1990, Pharmacological profile of BN50739, a new PAF antagonist in vitro and in vivo. Prostaglandins39: 469–480.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Tian-Li Yue
    • 1
  • Paul G. Lysko
    • 1
  • Giora Feuerstein
    • 1
  1. 1.Department of PharmacologySmithKline Beecham PharmaceuticalsKing of PrussiaUSA

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