Preparation and Properties of Monomethoxypoly(Ethylene Glycol)-Modified Enzymes for Therapeutic Applications

  • F. M. Veronese
  • P. Caliceti
  • O. Schiavon
  • L. Sartore
Part of the Topics in Applied Chemistry book series (TAPP)


The therapeutic value of enzymes as drugs would be considerably increased if drawbacks such as immunogenicity and antigenicity, rapid clearance from circulation, difficulty in targeting, instability, and inadequate supply were overcome. Genetic engineering seems to be promising in obtaining large amounts of useful enzymes, although doubts exist concerning the correct folding of expressed proteins.1 Nevertheless, the disadvantages of limited tissue distribution and rapid clearance from circulation of enzymes remain a major problem. Moreover, genetic methods cannot be used for producing enzymes carrying post-transcriptional modifications, but with this aim the so-called transgenic animal technology appears to be quite promising. For these reasons, alternative strategies are being actively investigated in several laboratories. These strategies include surface modification of the enzymes by chemical modification or compartmentalization of the enzyme onto complex structures which isolate it from body cells, tissues, and proteolytic enzymes.23


Protein Modification Affinity Constant Rapid Clearance Guanidinium Chloride Radioactive Amino Acid 
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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • F. M. Veronese
    • 1
  • P. Caliceti
    • 1
  • O. Schiavon
    • 1
  • L. Sartore
    • 1
  1. 1.Department of Pharmaceutical Sciences (Centro di Studio di Chimica del Farmaco e dei Prodotti Biologicamente Attivi del CNR)University of PaduaPaduaItaly

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